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GFP抗体

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX13970
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

目录号 GTX13970

文献引用 38

宿主

 Chicken

克隆

 Polyclonal

同种型

 IgY

实验应用

 WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC, IHC (Free Floating)

种属反应

 Species independent
产品包装
100 μl (¥5000)
查看所有 GFP 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:10000
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
ELISA Assay dependent
IHC 1:2000-1:5000
IHC (Free Floating) Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

Sodium Phosphate (10 mM, pH7.2) buffered isotonic saline (0.9%), 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

浓度

10 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant.

纯化方式

Purified by antigen-affinity chromatography
Serile filtered.

偶联

Unconjugated

RRID

AB_371416

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Green Fluorescent Protein

功能与背景

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

研究领域

產品图片

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

IHC analysis of paraformaldehyde-fixed (4%) neonatal mouse brain using GTX13970 GFP antibody.

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX13970

GTX13970 IHC-Wm Image

The data was published in the journal Elife in 2017.PMID: 28832322

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

The data was published in the journal Sci Rep in 2017. PMID: 28959031

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

The data was published in the journal Elife in 2014. PMID: 25490154

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

文献引用

Application Reference

Williamson MR et al. Nat Commun 2023; 14 (1) : 6341 Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke.
X Meng et al. Nature 2023; Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment
实验应用 : ICC/IF
Uttley K et al. Life Sci Alliance 2023; 6 (11) Unique activities of two overlapping PAX6 retinal enhancers.
Jin C et al. Bioeng Transl Med 2023; 8 (2) : e10448 Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.
Abdel-Razek O et al. Front Mol Biosci 2023; 10 : 1292076 Calcium signaling mediates proliferation of the precursor cells that give rise to the ciliated left-right organizer in the zebrafish embryo.
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Application Reference
Williamson MR et al. Nat Commun 2023; 14 (1):6341 Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke.
X Meng et al. Nature 2023; Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment
实验应用 : ICC/IF
Uttley K et al. Life Sci Alliance 2023; 6 (11) Unique activities of two overlapping PAX6 retinal enhancers.
Jin C et al. Bioeng Transl Med 2023; 8 (2):e10448 Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.
Abdel-Razek O et al. Front Mol Biosci 2023; 10:1292076 Calcium signaling mediates proliferation of the precursor cells that give rise to the ciliated left-right organizer in the zebrafish embryo.
Trolese MC et al. Mol Ther 2022; 30 (8):2760-2784 Boosting the peripheral immune response in the skeletal muscles improved motor function in ALS transgenic mice.
Kim S et al. Neuron 2022; 110 (8):1371-1384.e7 Co-packaging of opposing neurotransmitters in individual synaptic vesicles in the central nervous system.
实验应用 : IHC
Revah O et al. Nature 2022; 610 (7931):319-326 Maturation and circuit integration of transplanted human cortical organoids.
Domenico M Guarascio et al. J Physiol 2021; 599 (15):3697-3714 Functional expression of TMEM16A in taste bud cells.
实验应用 : IHC-Fr
Peu Santra et al. Dis Model Mech 2021; 14 (7) Loss of vacuolar-type H+-ATPase induces caspase-independent necrosis-like death of hair cells in zebrafish neuromasts.
实验应用 : IHC
Takahashi N et al. Curr Biol 2021; 31 (18):4148-4155.e4 Thalamic input to motor cortex facilitates goal-directed action initiation.
Kim S. et al. bioRxiv 2021; (Epub) Biophysical demonstration of co-packaging of glutamate and GABA in individual synaptic vesicles in the central nervous system.
DeMarco E et al. Front Neuroanat 2021; 15:636683 Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis.
实验应用 : IHC-Fr
Yuki Miura et al. Nat Biotechnol 2020; 12 (1421-1430) Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.
实验应用 : ICC/IF
Granger AJ et al. Elife 2020; 9 Cortical ChAT+ neurons co-transmit acetylcholine and GABA in a target- and brain-region-specific manner.
Gebhardt C et al. Nat Commun 2019; 10 (1):5471 An interhemispheric neural circuit allowing binocular integration in the optic tectum.
DeMarco E et al. J Comp Neurol 2019; (Epub) Neuron types in the zebrafish optic tectum labeled by an id2b transgene.
实验应用 : IHC
Bercier V et al. Mol Neurodegener 2019; 14 (1):27 Dynactin1 depletion leads to neuromuscular synapse instability and functional abnormalities.
Barth AIM et al. PLoS One 2018; 13 (10):e0204957 Regulation of epithelial migration by epithelial cell adhesion molecule requires its Claudin-7 interaction domain.
实验应用 : ICC/IF
Yao X et al. Dev Cell 2018; 45 (4):526-536.e5 Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells.
实验应用 : ICC/IF
Zanini D et al. Neuron 2018; (Epub) Proprioceptive Opsin Functions in Drosophila Larval Locomotion.
Birey F et al. Nature 2018; (Epub) Assembly of functionally integrated human forebrain spheroids.
实验应用 : ICC/IF, IHC-Fr
Di Donato V et al. Neuron 2018; (Epub) An Attractive Reelin Gradient Establishes Synaptic Lamination in the Vertebrate Visual System.
Shi Y et al. Nat Med 2018; (Epub) Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.
实验应用 : ICC/IF
Hsu WH et al. Sci Rep 2017; 7 (1):12386 COUP-TFII is required for morphogenesis of the neural crest-derived tympanic ring.
Ciarlo C et al. Elife 2017; 6 A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development.
Abraira VE et al. Cell 2017; 168 (43102):295-310.e19 The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn.
Micheva K.D et al. Cellular Imaging 2017; (Epub):149-182 Conjugate Immunofluorescence—SEM Array Tomography for Studying Mammalian Synapses and Axons.
Birey F et al. Nature 2017; 545 (7652):54-59 Assembly of functionally integrated human forebrain spheroids.
实验应用 : IHC-Fr
Henson MA et al. Neurobiol Learn Mem 2016; (Epub) Long-term depression-associated signaling is required for an in vitro model of NMDA receptor-dependent synapse pruning.
实验应用 : ICC/IF
Collman F et al. J Neurosci 2015; 35 (14):5792-807 Mapping synapses by conjugate light-electron array tomography.
Matsuyama M et al. Proc Natl Acad Sci U S A 2015; 112 (22):E2947-56 Avian sarcoma leukosis virus receptor-envelope system for simultaneous dissection of multiple neural circuits in mammalian brain.
Auer TO et al. Elife 2015; 4 (Epub) Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3.
Saunders A et al. Nature 2015; 521 (7550):31291 A direct GABAergic output from the basal ganglia to frontal cortex.
实验应用 : ICC/IF
Julia L. Semmelhack et al. Elife 2014; 3 A dedicated visual pathway for prey detection in larval zebrafish.
实验应用 : IHC-Wm
Thiele TR et al. Neuron 2014; (Epub) Descending Control of Swim Posture by a Midbrain Nucleus in Zebrafish.
实验应用 : IHC-Wm
Micheva KD et al. Cold Spring Harb Protoc 2010; 2010 (11):pdb.prot5526 Array tomography: imaging stained arrays.
Wyart C et al. Nature 2009; 461 (7262):407-10 Optogenetic dissection of a behavioural module in the vertebrate spinal cord.
实验应用 : IHC
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 5000
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