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GFP抗体

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Western Blot (WB). GTX20290

目录号 GTX20290

文献引用 20

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IHC, IM, IHC (Free Floating)

种属反应

 Species independent
产品包装
50 μl (¥5300)
查看所有 GFP 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
IHC Assay dependent
IM Assay dependent
IHC (Free Floating) Assay dependent
以下为常规应用缩写的中文注解
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

产品属性

形式

Liquid

存储溶液

Serum

保存剂

0.01% Thimerosal

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

免疫原种属

Aequorea victoria

免疫原

The GFP was isolated directly from the jellyfish Aequorea victoria.

纯化方式

Unpurified

偶联

Unconjugated

RRID

AB_371415

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

研究领域

產品图片

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal J Neurochem in 2018. PMID: 29178418

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal EMBO J in 2019. PMID: 31368584

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal EMBO J in 2019. PMID: 31368584

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal EMBO J in 2019. PMID: 31368584

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal EMBO J in 2019. PMID: 31368584

Anti-GFP antibody used in Western Blot (WB). GTX20290

GTX20290 WB Image

The data was published in the journal PLoS One in 2013. PMID: 23737990

文献引用

Application Reference

Thosar SA et al. Cell Rep 2024; 43 (1) : 113656 Oxidative guanine base damage plays a dual role in regulating productive ALT-associated homology-directed repair.
Laspata N et al. Nucleic Acids Res 2023; 51 (5) : 2215-2237 PARP1 associates with R-loops to promote their resolution and genome stability.
实验应用 : WB
Boris Simonetti et al. PLoS Biol 2022; 20 (4) : e3001601 SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling.
实验应用 : WB
Boris Simonetti et al. Proc Natl Acad Sci U S A 2022; 119 (25) : e2201980119 ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1.
实验应用 : WB
van Berkel AA et al. J Neurochem 2021; 157 (3) : 450-466 Loss of MUNC18-1 leads to retrograde transport defects in neurons.
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Application Reference
Thosar SA et al. Cell Rep 2024; 43 (1):113656 Oxidative guanine base damage plays a dual role in regulating productive ALT-associated homology-directed repair.
Laspata N et al. Nucleic Acids Res 2023; 51 (5):2215-2237 PARP1 associates with R-loops to promote their resolution and genome stability.
实验应用 : WB
Boris Simonetti et al. PLoS Biol 2022; 20 (4):e3001601 SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling.
实验应用 : WB
Boris Simonetti et al. Proc Natl Acad Sci U S A 2022; 119 (25):e2201980119 ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1.
实验应用 : WB
van Berkel AA et al. J Neurochem 2021; 157 (3):450-466 Loss of MUNC18-1 leads to retrograde transport defects in neurons.
Borga C et al. Leukemia 2019; 33 (2):333-347 Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis.
Magic Z et al. Int J Oncol 2019; 55 (6):1275-1286 Ephrin?2 inhibits cell proliferation and motility in?vitro and predicts longer metastasis?ree survival in breast cancer.
Zeljana Magic et al. International Journal of Oncology 2019; (Epub) Ephrin?B2 inhibits cell proliferation and motility in vitro and predicts longer metastasis?free survival in breast cancer.
Brouwer M et al. EMBO J 2019;:e101289 SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering.
实验应用 : ICC/IF, IP
Fouquerel E et al. Mol Cell 2019; (Epub) Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis.
Scanlon DP et al. Nat Commun 2017; 8:15220 An evolutionary switch in ND2 enables Src kinase regulation of NMDA receptors.
Dominguez N et al. J Neurochem 2017; (Epub) Dense-Core Vesicle biogenesis and exocytosis in neurons lacking Chromogranins A and B.
Wallez Y et al. J Biol Chem 2014; 289 (15):10431-44 Association of the Breast Cancer Antiestrogen Resistance Protein 1 (BCAR1) and BCAR3 Scaffolding Proteins in Cell Signaling and Antiestrogen Resistance.
实验应用 : WB, IP
Ramos-Ibeas P et al. Reproduction 2013; 146 (5):481-9 Most regions of mouse epididymis are able to phagocytose immature germ cells.
实验应用 : ICC/IF
Bordignon V et al. PLoS One 2013; 8 (5):e64613 Production of cloned pigs with targeted attenuation of gene expression.
实验应用 : WB
Mace PD et al. Nat Struct Mol Biol 2011; 18 (12):1381-7 NSP-Cas protein structures reveal a promiscuous interaction module in cell signaling.
实验应用 : IP
Kramer LB et al. PLoS One 2010; 5 (12):e14291 UEV-1 is an ubiquitin-conjugating enzyme variant that regulates glutamate receptor trafficking in C. elegans neurons.
实验应用 : WB
Liu FJ et al. PLoS One 2010; 5 (6):e11152 The Werner syndrome protein suppresses telomeric instability caused by chromium (VI) induced DNA replication stress.
实验应用 : ICC/IF
Catalucci D et al. J Cell Biol 2009; 184 (6):923-33 Akt regulates L-type Ca2+ channel activity by modulating Cavalpha1 protein stability.
Dunlap SM et al. Proc Natl Acad Sci U S A 2007; 104 (28):11736-41 *-like growth factor binding protein 2 promotes glioma development and progression.
实验应用 : IHC
SDS
Thimerosal.pdf
产品包装 报价 (¥)
¥ 5300
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