产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2
预测种属反应
Mouse, Rat, Zebrafish, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.45 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human GM130. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886243
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
golgin A2 , GM130
细胞定位
Golgi apparatus , Golgi stack membrane; Peripheral membrane protein
功能与背景
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX130351 ICC/IF Image
GM130 antibody detects GM130 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GM130 protein stained by GM130 antibody (GTX130351) diluted at 1:200.
Blue: Hoechst 33342 staining.
|
|
|
GTX130351 IP Image
Immunoprecipitation of GM130 protein from HeLa whole cell extracts using 5 μg of GM130 antibody (GTX130351).
Western blot analysis was performed using GM130 antibody (GTX130351).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
|
|
|
GTX130351 WB Image
GM130 antibody detects GM130 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GM130 antibody (GTX130351) diluted by 1:1000.
|
文献引用
产品评论
目前没有使用 GM130 antibody (GTX130351) 的产品评论被收录。快发表第一篇产品评论吧！
