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GOLGA5抗体 [N2C2], Internal

Anti-GOLGA5 antibody [N2C2], Internal used in Western Blot (WB). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in Western Blot (WB). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255

目录号 GTX104255

文献引用 4
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Hamster
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

83 kDa. ( Note )

阳性对照

Mouse brain , A549

预测种属反应

Rat, Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2037117

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

golgin A5 , GOLIM5 , RFG5 , ret-II

细胞定位

Golgi apparatus membrane; Single-pass type IV membrane protein

功能与背景

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-GOLGA5 antibody [N2C2], Internal used in Western Blot (WB). GTX104255

GTX104255 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX104255 diluted at 1:1000

Anti-GOLGA5 antibody [N2C2], Internal used in Western Blot (WB). GTX104255

GTX104255 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX104255 diluted at 1:1000

Anti-GOLGA5 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104255

GTX104255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human smooth muscle, using GOLGA5(GTX104255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255

GTX104255 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLGA5(GTX104255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255

GTX104255 ICC/IF Image

The data was published in the journal Sci Rep in 2017. PMID: 28615694

Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255

GTX104255 ICC/IF Image

The data was published in the journal Wellcome Open Res in 2019.PMID: 32030357

文献引用

Application Reference

P?rez-Rodriguez S et al. PLoS One 2020; 15 (8) : e0237930 Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis.
实验应用 : WB
种属反应 : Hamster
Popa S et al. Wellcome Open Res 2019; 4 : 119 Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion.
Chang HY et al. Sci Rep 2017; 7 (1) : 3515 Selective serotonin reuptake inhibitor, fluoxetine, impairs E-cadherin-mediated cell adhesion and alters calcium homeostasis in pancreatic beta cells.
实验应用 : ICC/IF
种属反应 : Mouse
Reiling JH et al. Nat Cell Biol 2013; 15 (12) : 1473-85 A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.
实验应用 : WB
种属反应 : Human
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产品评论

GOLGA5 antibody [N2C2], Internal 目录号 GTX104255

Rating
( Average 4 based on 2 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 2 users reviews)
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255
Date : Anonymous submitted on 21-Dec-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA, 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:100, 4°C, 16Hr
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HaCaT cells
Blocking : 2% FBS in PBS, 37°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.1% Triton X-100, 2% FBS in PBS, 25°C, 30minHr
Primary Antibody : 1:500, 4°C, 16Hr
我要评论产品
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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