GOLPH2抗体 [C3], C-term

Name: GOLPH2抗体 [C3], C-term
Brand: GeneTex
SKU: GTX107702
Price: 4000.00 RMB

Anti-GOLPH2 antibody [C3], C-term used in Western Blot (WB). GTX107702

Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702

Anti-GOLPH2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107702

目录号 GTX107702

文献引用 5

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P
种属反应	Human
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX107702 on CiteAb

产品说明

摘要

Golgi membrane protein 1, also known as GP73, GOLM1, and GOLPH2, is a type II Golgi membrane glycoprotein with a predicted molecular weight of 45 kDa. GOLPH2 is often overexpressed in the hepatocytes of patients with hepatitis and hepatocellular carcinoma (HCC). Reports indicate that GOLPH2 is a promising serum marker for the diagnosis of HCC.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:5000
IHC-P	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

45 kDa. ( Note )

阳性对照

HeLa membrane fraction , DU145 , 293T

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GOLPH2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240906

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

golgi membrane protein 1 , C9orf155 , GOLPH2 , GP73 , HEL46 , PSEC0257 , bA379P1.3

细胞定位

Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein

功能与背景

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

数据库

Gene ID: 51280 GOLM1
UniProt: Q8NBJ4 GOLM1

研究领域

產品图片

GTX107702 WB Image

Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107702 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107702 WB Image

GOLPH2 antibody detects GOLPH2 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa membrane fraction lysate/extract
10 % SDS-PAGE
GOLPH2 antibody (GTX107702) dilution: 1:500

GTX107702 ICC/IF Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:5000.
Red: Phalloidin, a F-actin marker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107702 ICC/IF Image

Immunofluorescence analysis of 70%methanol,30% Acetone-fixed HCT116 , using GOLPH2(GTX107702) antibody at 1:200 dilution.

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
GOLPH2 antibody [C3], C-term (GTX107702) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107702 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GOLPH2(GTX107702) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

文献引用

Application Reference

Chua HH et al. Cell Mol Gastroenterol Hepatol 2023; 15 (1) : 121-152 PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.

Truberg J et al. Biochim Biophys Acta Mol Cell Res 2022; 1869 (11) : 119345 Endogenous tagging reveals a mid-Golgi localization of the glycosyltransferase-cleaving intramembrane protease SPPL3

实验应用 : WB

种属反应 : Human

Inoue J et al. J Virol 2019; (Epub) siRNA Screening for the Small GTPase Rab Proteins Identifies that Rab5B is a Major Regulator of Hepatitis B Virus Production.

实验应用 : WB

种属反应 : Human

Ding X et al. Brain Res 2019; 1717 : 117-126 GOLM1 silencing inhibits the proliferation and motility of human glioblastoma cells via the Wnt/β-catenin signaling pathway.

实验应用 : IHC-P

种属反应 : Human

Liu KK et al. Autophagy 2016; 13 (1) : 187-200 Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway.

实验应用 : ICC/IF

种属反应 : Human

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Application Reference

Chua HH et al. Cell Mol Gastroenterol Hepatol 2023; 15 (1):121-152 PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.

Truberg J et al. Biochim Biophys Acta Mol Cell Res 2022; 1869 (11):119345 Endogenous tagging reveals a mid-Golgi localization of the glycosyltransferase-cleaving intramembrane protease SPPL3

实验应用 : WB

种属反应 : Human

Inoue J et al. J Virol 2019; (Epub) siRNA Screening for the Small GTPase Rab Proteins Identifies that Rab5B is a Major Regulator of Hepatitis B Virus Production.

实验应用 : WB

种属反应 : Human

Ding X et al. Brain Res 2019; 1717:117-126 GOLM1 silencing inhibits the proliferation and motility of human glioblastoma cells via the Wnt/β-catenin signaling pathway.

实验应用 : IHC-P

种属反应 : Human

Liu KK et al. Autophagy 2016; 13 (1):187-200 Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway.

实验应用 : ICC/IF

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
	genetex@neobioscience.com

GOLPH2抗体 [C3], C-term

目录号 GTX107702

宿主

克隆

同种型

实验应用

种属反应

摘要

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX107702 WB Image

GTX107702 IHC-P Image

GTX107702 IHC-P Image

GTX107702 WB Image

GTX107702 WB Image

GTX107702 ICC/IF Image

GTX107702 IHC-P Image

GTX107702 ICC/IF Image

GTX107702 IHC-P Image

GTX107702 ICC/IF Image

文献引用

Application Reference

产品评论

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