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GOLPH2抗体

KO/KD Orthogonal
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154
Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116154
Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154
Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

目录号 GTX116154

文献引用 2
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

45 kDa. ( Note )

阳性对照

DU145 , HeLa membrane extract , 293T

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human GOLPH2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11171591

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

golgi membrane protein 1 , C9orf155 , GOLPH2 , GP73 , HEL46 , PSEC0257 , bA379P1.3

细胞定位

Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein

功能与背景

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116154

GTX116154 IHC-P Image

GOLPH2 antibody detects GOLPH2 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
GOLPH2 stained by GOLPH2 antibody (GTX116154) diluted at 1:10000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154

GTX116154 ICC/IF Image

GOLPH2 antibody detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOLPH2 stained by GOLPH2 antibody (GTX116154) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

KO/KD

Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154

GTX116154 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLPH2(GTX116154) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Truberg J et al. Biochim Biophys Acta Mol Cell Res 2022; 1869 (11) : 119345 Endogenous tagging reveals a mid-Golgi localization of the glycosyltransferase-cleaving intramembrane protease SPPL3
实验应用 : WB
种属反应 : Human
Yoon MJ et al. Carcinogenesis 2013; 34 (8) : 1918-28 Monensin, a polyether ionophore antibiotic, overcomes TRAIL resistance in glioma cells via endoplasmic reticulum stress, DR5 upregulation and c-FLIP downregulation.
实验应用 : ICC/IF
种属反应 : Human
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产品评论

GOLPH2 antibody 目录号 GTX116154

Rating
( Average 5 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154
Date : Anonymous submitted on 27-Oct-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA and 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:100, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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