产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HepG2 , Neuro2A , rat lung , 293T
预测种属反应
Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1240908
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glutamic-oxaloacetic transaminase 2 , KAT4 , KATIV , KYAT4 , mitAAT
细胞定位
Mitochondrion matrix , Cell membrane
功能与背景
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX101929 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101929 WB Image
GOT2 antibody detects GOT2 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract 10% SDS-PAGE GOT2 antibody (GTX101929) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101929 IHC-P Image
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2(GTX101929) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101929 IHC-P Image
GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GOT2 antibody (GTX101929) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101929 IHC-P Image
GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. GOT2 antibody (GTX101929) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101929 IHC-P Image
GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GOT2 antibody (GTX101929) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101929 WB Image
Sample (30 μg of whole cell lysate) A: HepG2 10% SDS PAGE GTX101929 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101929 WB Image
GOT2 antibody detects GOT2 protein by western blot analysis. A. 50 μg rat lung lysate/extract 10% SDS-PAGE GOT2 antibody (GTX101929) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101929 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using GOT2(GTX101929) antibody at 1:500 dilution.
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文献引用
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