产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human GPD1 protein, and it does not cross-react with human GPDL1 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-term region of human GPD1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glycerol-3-phosphate dehydrogenase 1 , GPD-C , GPDH-C , HTGTI
细胞定位
Cytoplasm
功能与背景
This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
数据库
研究领域
產品图片
![Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796](/templates/default/js/lightbox/images/loading.gif "Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796")
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GTX637796 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (GTX637796) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637796 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (GTX637796) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637796 ICC/IF Image
GPD1 antibody [HL1959] detects GPD1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.Green: GPD1 stained by GPD1 antibody [HL1959] (GTX637796) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637796 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (GTX637796) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637796 IHC-P Image
GPD1 antibody [HL1959] detects GPD1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.GPD1 stained by GPD1 antibody [HL1959] (GTX637796) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796](/upload/website/prouct_img/small/GTX637796/GTX637796_T-44851_20230428_WB_multiple_B_23050223_653.webp "Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796")
![Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796](/upload/website/prouct_img/small/GTX637796/GTX637796_T-44851_20230512_WB_M_R_23051702_830.webp "Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796")
![Anti-GPD1 antibody [HL1959] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637796](/upload/website/prouct_img/small/GTX637796/GTX637796_45089_20231006_ICC_IF_23102401_538.webp "Anti-GPD1 antibody [HL1959] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637796")
![Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796](/upload/website/prouct_img/small/GTX637796/GTX637796_45089_20230707_WB_TPM_watermark_23071223_702.webp "Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796")
![Anti-GPD1 antibody [HL1959] used in IHC (Paraffin sections) (IHC-P). GTX637796](/upload/website/prouct_img/small/GTX637796/GTX637796_T-44851_20230801_IHC-P_M_23081619_919.webp "Anti-GPD1 antibody [HL1959] used in IHC (Paraffin sections) (IHC-P). GTX637796")
![Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796](/upload/website/prouct_img/normal/GTX637796/GTX637796_T-44851_20230428_WB_multiple_B_23050223_653.webp "Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796")
![Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796](/upload/website/prouct_img/normal/GTX637796/GTX637796_T-44851_20230512_WB_M_R_23051702_830.webp "Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796")
![Anti-GPD1 antibody [HL1959] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637796](/upload/website/prouct_img/normal/GTX637796/GTX637796_45089_20231006_ICC_IF_23102401_538.webp "Anti-GPD1 antibody [HL1959] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637796")
![Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796](/upload/website/prouct_img/normal/GTX637796/GTX637796_45089_20230707_WB_TPM_watermark_23071223_702.webp "Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796")
![Anti-GPD1 antibody [HL1959] used in IHC (Paraffin sections) (IHC-P). GTX637796](/upload/website/prouct_img/normal/GTX637796/GTX637796_T-44851_20230801_IHC-P_M_23081619_919.webp "Anti-GPD1 antibody [HL1959] used in IHC (Paraffin sections) (IHC-P). GTX637796")
