GPR50抗体 [HL4628]

GPR50 antibody [HL4628] detects GPR50 protein by immunofluorescent analysis.
Sample: NCI-H520 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPR50 stained by GPR50 antibody [HL4628] (GTX644492) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield™ with DAPI (GTX30920).

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR50 antibody [HL4628] (GTX644492) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled NCI-H520 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR50 antibody [HL4628] (GTX644492) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)