产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Bovine, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Ser670 of human GRK2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909912
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
G protein-coupled receptor kinase 2 , ADRBK1 , BARK1 , BETA-ARK1
细胞定位
Cytoplasm,Cell membrane
功能与背景
This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]
数据库
研究领域
產品图片
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/templates/default/js/lightbox/images/loading.gif "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
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GTX635875 WB Image
Various tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635875 WB Image
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635875 WB Image
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635875 IHC-P Image
GRK2 (phospho Ser670) antibody [HL1035] detects GRK2 (phospho Ser670) protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.GRK2 (phospho Ser670) stained by GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635875 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635875 WB Image
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635875 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/small/GTX635875/GTX635875_44200_20220701_WB_M_thymus_22070501_687.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/small/GTX635875/GTX635875_44200_20210122_WB_treatment_Pervanadate_w_23061202_652.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/small/GTX635875/GTX635875_T-44158_20210122_WB_treatment_CIP_w_23061202_800.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in IHC (Paraffin sections) (IHC-P). GTX635875](/upload/website/prouct_img/small/GTX635875/GTX635875_44200_20210226_IHC-P_M_w_23061202_147.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in IHC (Paraffin sections) (IHC-P). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/small/GTX635875/GTX635875_T-44158_20210122_WB_M_w_23061202_546.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/small/GTX635875/GTX635875_44200_20220128_WB_treatment_Pervanadate_peptideblocking_w_23061202_731.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/small/GTX635875/GTX635875_44200_20210910_WB_B_w_23061202_300.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/normal/GTX635875/GTX635875_44200_20220701_WB_M_thymus_22070501_687.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/normal/GTX635875/GTX635875_44200_20210122_WB_treatment_Pervanadate_w_23061202_652.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/normal/GTX635875/GTX635875_T-44158_20210122_WB_treatment_CIP_w_23061202_800.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in IHC (Paraffin sections) (IHC-P). GTX635875](/upload/website/prouct_img/normal/GTX635875/GTX635875_44200_20210226_IHC-P_M_w_23061202_147.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in IHC (Paraffin sections) (IHC-P). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/normal/GTX635875/GTX635875_T-44158_20210122_WB_M_w_23061202_546.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/normal/GTX635875/GTX635875_44200_20220128_WB_treatment_Pervanadate_peptideblocking_w_23061202_731.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
![Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875](/upload/website/prouct_img/normal/GTX635875/GTX635875_44200_20210910_WB_B_w_23061202_300.webp "Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875")
