GRP94抗体 [N1N3]

Name: GRP94抗体 [N1N3]
Brand: GeneTex
SKU: GTX103203
Price: 4000.00 RMB
Rating: 4 (1 reviews)

Anti-GRP94 antibody [N1N3] used in Western Blot (WB). GTX103203

Anti-GRP94 antibody [N1N3] used in Immunoprecipitation (IP). GTX103203

Anti-GRP94 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103203

Anti-GRP94 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103203

目录号 GTX103203

文献引用 8

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP
种属反应	Human, Mouse
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 GRP94 的产品

View product citations for antibody GTX103203 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

92 kDa. ( Note )

阳性对照

NIH-3T3 , H1299 , HeLaS3 , 293T

预测种属反应

Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human GRP94. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950517

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1

细胞定位

Endoplasmic reticulum lumen , Melanosome

功能与背景

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

数据库

Gene ID: 7184 HSP90B1
UniProt: P14625 HSP90B1

研究领域

產品图片

GTX103203 WB Image

Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody [N1N3] (GTX103203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103203 IP Image

Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody [N1N3] (GTX103203).
Western blot analysis was performed using GRP94 antibody [N1N3] (GTX103203).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103203 ICC/IF Image

GRP94 antibody [N1N3] detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody [N1N3] (GTX103203) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103203 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela S3
7.5% SDS PAGE
GTX103203 diluted at 1:1000

GTX103203 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft , using HSP90B(GTX103203) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103203 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX103203 diluted at 1:5000

GTX103203 WB Image

The data was published in the journal Front Neurol in 2019.PMID: 31258504

GTX103203 WB Image

The data was published in the journal Elife in 2016.PMID: 27435961

GTX103203 WB Image

The data was published in the 2022 in Orphanet J Rare Dis. PMID: 35101074

文献引用

Application Reference

Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

实验应用 : WB

种属反应 : Human

Arlt A et al. Orphanet J Rare Dis 2022; 17 (1) : 29 Novel insights into PORCN mutations, associated phenotypes and pathophysiological aspects.

实验应用 : WB

种属反应 : Human

Lee CW et al. Molecules 2022; 27 (2) : 373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

实验应用 : WB

种属反应 : Human

Gatz C et al. Front Neurol 2019; 10 : 562 Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sj?gren Syndrome.

实验应用 : WB

种属反应 : Human

Ram?rez-Peinado S et al. J Cell Sci 2017; 130 (14) : 2251-2265 TRAPPC13 modulates autophagy and the response to Golgi stress.

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产品评论

GRP94 antibody [N1N3] 目录号 GTX103203

Rating

( Average 4 based on 1 users reviews)

实验应用

Immunocytochemistry/ Immunofluorescence(ICC/IF)

( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 25-Dec-2014

Score :

Application Tested : ICC/IF

Sample Species : Hu

Sample : endothelial cells

Blocking : 10% goat serum in PBS, 25°C, 1Hr

Fixation : 4% PFA in PBS

Permeabilization /Antigen retrieval : 0.2% Triton X100, 25°C, 10minHr

Primary Antibody : 1:200, 4°C, 17Hr

我要评论产品

Application Reference

Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

实验应用 : WB

种属反应 : Human

Arlt A et al. Orphanet J Rare Dis 2022; 17 (1):29 Novel insights into PORCN mutations, associated phenotypes and pathophysiological aspects.

实验应用 : WB

种属反应 : Human

Lee CW et al. Molecules 2022; 27 (2):373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

实验应用 : WB

种属反应 : Human

Gatz C et al. Front Neurol 2019; 10:562 Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sj?gren Syndrome.

实验应用 : WB

种属反应 : Human

Ram?rez-Peinado S et al. J Cell Sci 2017; 130 (14):2251-2265 TRAPPC13 modulates autophagy and the response to Golgi stress.

Plate L et al. Elife 2016; 5 Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation.

实验应用 : WB

种属反应 : Human

Krishnan S et al. Virulence 2015; 6 (8):777-86 Serotype O18 avian pathogenic and neonatal meningitis Escherichia coli strains employ similar pathogenic strategies for the onset of meningitis.

Cooley CB et al. Proc Natl Acad Sci U S A 2014; 111 (36):13046-51 Unfolded protein response activation reduces secretion and extracellular aggregation of amyloidogenic immunoglobulin light chain.

SDS
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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GRP94抗体 [N1N3]

目录号 GTX103203

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX103203 WB Image

GTX103203 IP Image

GTX103203 ICC/IF Image

GTX103203 WB Image

GTX103203 IHC-P Image

GTX103203 WB Image

GTX103203 WB Image

GTX103203 WB Image

GTX103203 WB Image

文献引用

Application Reference

产品评论

GRP94 antibody [N1N3] 目录号 GTX103203

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