产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2 , NIH3T3
预测种属反应
Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950516
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1
细胞定位
Endoplasmic reticulum lumen , Melanosome
功能与背景
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]
数据库
研究领域
產品图片
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GTX103232 WB Image
Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103232 WB Image
GRP94 antibody detects GRP94 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000
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GTX103232 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94(GTX103232) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.
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GTX103232 IP Image
Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody (GTX103232).
Western blot analysis was performed using GRP94 antibody (GTX103232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX103232 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103232 WB Image
GRP94 antibody detects GRP94 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000
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GTX103232 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94(GTX103232) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103232 ICC/IF Image
GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody (GTX103232) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX103232 IHC-P Image
GRP94 antibody detects GRP94 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
GRP94 antibody (GTX103232) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103232 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25811758
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文献引用
产品评论
GRP94 antibody
目录号 GTX103232
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Rating
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( Average 5 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 05-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
Figure Note :
0-30 μM cisplatin treatment for 24hr.
