产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Sheep, Bovine, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human GSK3 beta. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888582
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glycogen synthase kinase 3 beta
细胞定位
Cytoplasm,Nucleus,Cell membrane
功能与背景
The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]
数据库
研究领域
產品图片
![Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816](/templates/default/js/lightbox/images/loading.gif "Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816")
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GTX635816 IHC-P Image
GSK3 beta antibody [GT16711] detects GSK3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.GSK3 beta stained by GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635816 ICC/IF Image
GSK3 beta antibody detects GSK3 beta protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSK3 beta stained by GSK3 beta antibody (GTX635816) diluted at 1:500.
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GTX635816 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635816 IHC-P Image
GSK3 beta antibody [GT16711] detects GSK3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.GSK3 beta stained by GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635816 WB Image
Wild-type (WT) and GSK3 beta knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT16711] (GTX635816) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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文献引用
产品评论
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![Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816](/upload/website/prouct_img/small/GTX635816/GTX635816_44118_20210222_IHC-P_M_w_23061202_221.webp "Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635816](/upload/website/prouct_img/small/GTX635816/GTX635816_44118_20210716_ICC_IF_w_23061202_911.webp "Anti-GSK3 beta antibody [GT16711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816](/upload/website/prouct_img/small/GTX635816/GTX635816_44118_20210205_WB_M_R_w_23061202_516.webp "Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816](/upload/website/prouct_img/small/GTX635816/GTX635816_44118_20210222_IHC-P_R_w_23061202_757.webp "Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816](/upload/website/prouct_img/small/GTX635816/GTX635816_44118_20201113_WB_KO_watermark_w_23061202_922.webp "Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816](/upload/website/prouct_img/normal/GTX635816/GTX635816_44118_20210222_IHC-P_M_w_23061202_221.webp "Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635816](/upload/website/prouct_img/normal/GTX635816/GTX635816_44118_20210716_ICC_IF_w_23061202_911.webp "Anti-GSK3 beta antibody [GT16711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816](/upload/website/prouct_img/normal/GTX635816/GTX635816_44118_20210205_WB_M_R_w_23061202_516.webp "Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816](/upload/website/prouct_img/normal/GTX635816/GTX635816_44118_20210222_IHC-P_R_w_23061202_757.webp "Anti-GSK3 beta antibody [GT16711] used in IHC (Paraffin sections) (IHC-P). GTX635816")
![Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816](/upload/website/prouct_img/normal/GTX635816/GTX635816_44118_20201113_WB_KO_watermark_w_23061202_922.webp "Anti-GSK3 beta antibody [GT16711] used in Western Blot (WB). GTX635816")
