产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500 - 1:2000 |
1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A synthesized peptide derived from human Phospho-GSK3 beta (Ser9).
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glycogen synthase kinase 3 beta , GSK3B , GSK3 beta
细胞定位
Cytoplasm,Nucleus,Cell membrane
功能与背景
The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]
数据库
研究领域
產品图片
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GTX00971 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta (phospho Ser9) antibody [GT1209] (GTX00971) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00971 WB Image
WB analysis of HeLa cell extracts with/without Calyculin A (100 nM, 37ºC, 30 min) treatment after serum-starvation overnight using GTX00971 GSK3 beta (phospho Ser9) antibody [GT1209]. Dilution : 1:1000 Loading : 25μg
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GTX00971 IHC-P Image
IHC-P analysis of human colon carcinoma tissue section using GTX00971 GSK3 beta (phospho Ser9) antibody [GT1209]. Dilution : 1:100
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GTX00971 WB Image
WB analysis of NIH-3T3 cell extracts with/without Calyculin A (100 nM, 37ºC, 30 min) treatment after serum-starvation overnight using GTX00971 GSK3 beta (phospho Ser9) antibody [GT1209]. Dilution : 1:1000 Loading : 25μg
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文献引用
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产品评论
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