GST tag抗体

IP analysis of recombinant proteins using GTX26633 GST tag antibody.
Lane 1 : Recombinant GST-Pin1 protein
Lane 2 : Recombinant GST-CDK4 protein
Lane 3 : Recombinant GST-Pin1 and GST-CDK4 protein
Loading : 25 μg

WB analysis of recombinant GST proteins using GTX26633 GST tag antibody.
Lane 1 : Protein ladder
Lane 2 : 0.1 μg
Lane 3 : 0.05 μg
Lane 4 : 0.02 μg
Lane 5 : Mock
Dilution : 1 μg/mL

WB analysis of various samples using GTX26633 GST tag antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : CHO-K1 whole cell lysate
Lane 4 : MDA-MB-231 whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : Jurkat whole cell lysate
Lane 7 : NIH-3T3 whole cell lysate
Lane 8 : E. coli HCP
Lane 9 : Flag-tag protein
Lane 10 : RFP
Lane 11 : GFP
Lane 12 : GST protein
Lane 13 : MBP
Dilution : 1 μg/mL

ELISA analysis of recombinant GST protein using serially diluted GTX26633 GST tag antibody.
Coating : 1 μg

Immunoprecipitation of anti-GST antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 μg per lane. Primary antibody: anti-GST 1:400 for overnight at 4ºC. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC.

GeneTex anti-GST polyclonal antibody (GTX26633) in western blot shows detection of recombinant GST (indicated by band at ~ 28 kDa). The SDS-PAGE contained approximately 0.2 μg of rGST loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was transferred to nitrocellulose and blocked with “Blocking Buffer for Fluorescent Western Blotting” in TBS for 1h at RT. The membrane was probed with anti-GST antibody at a 1:2,000 dilution in blocking reagent, overnight at 4º C. For detection DyLight™800 conjugated Donkey-a-Goat IgG was used at a 1:20,000 dilution (in blocking reagent) for 30 min at 25º C.