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GSTT1抗体

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250
Anti-GSTT1 antibody used in Western Blot (WB). GTX109250
Anti-GSTT1 antibody used in Immunoprecipitation (IP). GTX109250
Anti-GSTT1 antibody used in Western Blot (WB). GTX109250
Anti-GSTT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109250
Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

目录号 GTX109250

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, IHC-P, IP

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

27 kDa. ( Note )

阳性对照

rat liver

预测种属反应

Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human GSTT1 Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950469

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glutathione S-transferase theta 1

细胞定位

Cytoplasm

功能与背景

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

GTX109250 WB Image

GSTT1 antibody detects GSTT1 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with GSTT1 antibody (GTX109250) diluted by 1:1000.

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

GTX109250 WB Image

GSTT1 antibody detects GSTT1 protein by western blot analysis.
A. 50 μg rat liver lysate/extract
12% SDS-PAGE
GSTT1 antibody (GTX109250) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTT1 antibody used in Immunoprecipitation (IP). GTX109250

GTX109250 IP Image

Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 μg of GSTT1 antibody (GTX109250).
Western blot analysis was performed using GSTT1 antibody (GTX109250).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

GTX109250 WB Image

GSTT1 antibody detects GSTT1 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTT1 antibody (GTX109250) diluted by 1:1000.

Anti-GSTT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109250

GTX109250 IHC-P Image

GSTT1 antibody detects GSTT1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
GSTT1 antibody (GTX109250) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

GTX109250 WB Image

The data was published in the journal Nat Commun in 2018. PMID: 30446643

文献引用

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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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