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Galectin 3抗体 [HL2020]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Anti-Galectin 3 antibody [HL2020] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637921
Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921
Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921

目录号 GTX637921

1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2020

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

26 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human Galectin 3

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

细胞定位

Cytoplasm,Nucleus,Secreted

功能与背景

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

数据库

研究领域

產品图片

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

GTX637921 WB Image

KO/KD

Wild-type (WT) and LGALS3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

GTX637921 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2020] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637921

GTX637921 ICC/IF Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921

GTX637921 IHC-P Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

GTX637921 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

GTX637921 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921

GTX637921 IHC-P Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat stomach.
Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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产品评论

Galectin 3 antibody [HL2020] 目录号 GTX637921

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : Calu-3 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 16Hr
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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