产品说明
摘要
Gli1 antibody detects Gli1 protein, a zinc-finger protein with a predicted molecular weight of 119 kDa. Gli1 is a transcription factor that serves as an effector of Sonic hedgehog signaling in mediating cell fate during embryonic development. In addition, Gli1 has been shown to control many basic cell functions including the cell cycle, growth, and cell death. Although Gli1 was originally isolated from a glioma tumor, its upregulation is found in many other tumor types.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Gli1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888543
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
GLI-Kruppel family member GLI1 , AV235269 , Zfp-5 , Zfp5
细胞定位
Cytoplasm,Nucleus
数据库
研究领域
產品图片
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/templates/default/js/lightbox/images/loading.gif "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
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GTX635619 WB Image
Non-transfected (–) and transfected (+) RMS13 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635619 IHC-P Image
Gli1 antibody [HL247] detects Gli1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635619 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635619 ICC/IF Image
Gli1 antibody [HL247] detects Gli1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX635619 IHC-P Image
Gli1 antibody [HL27] detects Gli1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Gli1 stained by Gli1 antibody [HL27] (GTX635619) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635619 WB Image
RMS 13 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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GTX635619 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44020_20201008_WB_shRNA_watermark_w_23061202_411.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44020_20200814_IHC-P_M_w_23061202_168.webp "Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44020_20210924_WB_B_w_23061202_513.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44550_20230721_ICC_IF_B_23072519_127.webp "Anti-Gli1 antibody [HL247] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635619")
![Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44648_20221227_IHC-P_multiple_R_22122821_716.webp "Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44648_20231013_WB_Fraction_23101921_286.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44648_20220422_WB_22080923_776.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20201008_WB_shRNA_watermark_w_23061202_411.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20200814_IHC-P_M_w_23061202_168.webp "Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20210924_WB_B_w_23061202_513.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44550_20230721_ICC_IF_B_23072519_127.webp "Anti-Gli1 antibody [HL247] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635619")
![Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44648_20221227_IHC-P_multiple_R_22122821_716.webp "Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44648_20231013_WB_Fraction_23101921_286.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44648_20220422_WB_22080923_776.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
