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Gli2抗体

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in Western Blot (WB). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in Western Blot (WB). GTX27195

目录号 GTX27195

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, IHC-Fr, ELISA, IHC

种属反应

 Human, Mouse
产品包装
50 μg (¥5300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:2000
IHC-P 2-20 μg/mL
IHC-Fr Assay dependent
ELISA 1:15000-1:60000
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

165 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

20mM Potassium Phosphate, 150mM NaCl

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.

纯化方式

Purified by antigen-affinity chromatography.
From serum

偶联

Unconjugated

RRID

AB_386083

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

GLI-Kruppel family member GLI2

细胞定位

Nucleus,Cytoplasm

功能与背景

This protein belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

数据库

研究领域

產品图片

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195

GTX27195 IHC-P Image

GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195

GTX27195 IHC-P Image

GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain. Tissue was formalin-fixed and paraffin embedded.

Anti-Gli2 antibody used in Western Blot (WB). GTX27195

GTX27195 WB Image

Western blot using GeneTex's Affinity Purified anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). ~ 25 μg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750. Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDyeR 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown (M) using the 700 nm channel (red). IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195

GTX27195 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX27195 Gli2 antibody.
Dilution : 10 μg/mL

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195

GTX27195 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX27195 Gli2 antibody.
Dilution : 10 μg/mL

Anti-Gli2 antibody used in Western Blot (WB). GTX27195

GTX27195 WB Image

WB analysis of mouse brain tissue lysate using GTX27195 Gli2 antibody prior incubated with (lane 2) or without (lane 1) blocking peptide.
Loading : 25 μg
Dilution : 1:750

文献引用

Application Reference

Morgan H et al. Methods Mol Biol 2018; (Epub) Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells.
实验应用 : IHC-Fr
种属反应 : Human
Ma X et al. Neuroreport 2017; 28 (3) : 141-148 Crosstalk between Notch and Sonic hedgehog signaling in a mouse model of amyotrophic lateral sclerosis.
Colmont CS et al. Proc Natl Acad Sci U S A 2013; 110 (4) : 1434-9 CD200-expressing human basal cell carcinoma cells initiate tumor growth.
实验应用 : IHC
种属反应 : Human
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产品包装 报价 (¥)
¥ 5300
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