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Glutaminase C (GAC)抗体

KO/KD
Anti-Glutaminase C (GAC) antibody used in Western Blot (WB). GTX131263
Anti-Glutaminase C (GAC) antibody used in Immunoprecipitation (IP). GTX131263
Anti-Glutaminase C (GAC) antibody used in IHC (Paraffin sections) (IHC-P). GTX131263
Anti-Glutaminase C (GAC) antibody used in IHC (Paraffin sections) (IHC-P). GTX131263
Anti-Glutaminase C (GAC) antibody used in IHC (Paraffin sections) (IHC-P). GTX131263
Anti-Glutaminase C (GAC) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131263

目录号 GTX131263

文献引用 4
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Glutaminase C 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

65 kDa. ( Note )

阳性对照

MDA-MB-231

产品说明

Specific for GAC but not KGA isoform

预测种属反应

Rat, Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase C (GAC). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886452

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA

细胞定位

Mitochondrion

功能与背景

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

数据库

研究领域

產品图片

Anti-Glutaminase C (GAC) antibody used in Western Blot (WB). GTX131263

GTX131263 WB Image

KO/KD

Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX131263) at a dilution of 1:5000.
This antibody specifically recognizes GAC but not the KGA isoform

Anti-Glutaminase C (GAC) antibody used in Immunoprecipitation (IP). GTX131263

GTX131263 IP Image

Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of Glutaminase C antibody (GTX131263) or Glutaminase C antibody (GTX132402).
Western blot analysis was performed using Glutaminase C antibody (GTX131263).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Glutaminase C (GAC) antibody used in IHC (Paraffin sections) (IHC-P). GTX131263

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutaminase C (GAC) antibody used in IHC (Paraffin sections) (IHC-P). GTX131263

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on mouse duidenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duidenum.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutaminase C (GAC) antibody used in IHC (Paraffin sections) (IHC-P). GTX131263

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutaminase C (GAC) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131263

GTX131263 ICC/IF Image

Glutaminase C antibody detects Glutaminase C protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutaminase C protein stained by Glutaminase C antibody (GTX131263) diluted at 1:500.
Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

文献引用

Application Reference

Fazal S et al. Hum Mol Genet 2023; 32 (1) : 46-54 Repeat expansions nested within tandem CNVs: a unique structural change in GLS exemplifies the diagnostic challenges of non-coding pathogenic variation.
Barreca F et al. Antioxidants (Basel) 2023; 12 (8) SIRT5 Activation and Inorganic Phosphate Binding Reduce Cancer Cell Vitality by Modulating Autophagy/Mitophagy and ROS.
F. Barreca et al. Preprints.org 2023; : 2023052144 SIRT5 activation and inorganic phosphate binding reduce can- 2 cer cells vitality by modulating autophagy/mitophagy and 3 ROS.
实验应用 : ICC/IF
种属反应 : Human
Saladini S et al. Cells 2019; 8 (1)
实验应用 : WB
种属反应 : Human
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产品评论

Glutaminase C (GAC) antibody 目录号 GTX131263

Rating
( Average 4 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-Glutaminase C (GAC) antibody used in IHC (Paraffin sections) (IHC-P). GTX131263
Date : Anonymous submitted on 19-Mar-2015
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : cholangiocarcinoma tissue
Primary Antibody : 1:500, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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