产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2 , mouse eye , 293T , DDDDK-tagged GLUL-transfected 293T
预测种属反应
Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human Glutamine synthetase Recombinant protein.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888231
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59
细胞定位
Cytoplasm
功能与背景
Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]
数据库
研究领域
產品图片
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Frozen sections) (IHC-Fr). GTX630657](/templates/default/js/lightbox/images/loading.gif "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Frozen sections) (IHC-Fr). GTX630657")
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GTX630657 IHC-Fr Image
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX630657 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:500.
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GTX630657 IHC-P Image
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX630657 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX630657) diluted at 1:1000.
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GTX630657 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:250000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX630657 ICC/IF Image
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein in glial cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron & glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody (GTX130462) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX630657 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX630657 WB Image
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT7711] (GTX630657) dilution: 1:1000
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GTX630657 IHC-P Image
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX630657 IHC-P Image
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX630657 IHC-P Image
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:200.Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Frozen sections) (IHC-Fr). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_41617_20160824_IHC-Fr_w_23061202_803.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Frozen sections) (IHC-Fr). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_41617_20160630_WB_shRNA_watermark_w_23061202_443.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_44510_20231201_IHC-P_R_24010223_252.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_41617_20160825_WB_M_eye_w_23061202_230.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_44510_20211231_WB_M_R_w_23061202_308.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_44510_20220128_ICC_IF_w_23061202_442.webp "Anti-Glutamine synthetase antibody [GT7711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_41617_20180928_WB_B_w_23061202_816.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_41617_WB_w_23061202_387.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_44510_20231201_IHC-P_M_24010223_628.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_44825_20230609_IHC-P_multiple_M_23062718_255.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657](/upload/website/prouct_img/small/GTX630657/GTX630657_44825_20230609_IHC-P_M_1_23062718_893.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Frozen sections) (IHC-Fr). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_41617_20160824_IHC-Fr_w_23061202_803.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Frozen sections) (IHC-Fr). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_41617_20160630_WB_shRNA_watermark_w_23061202_443.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_44510_20231201_IHC-P_R_24010223_252.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_41617_20160825_WB_M_eye_w_23061202_230.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_44510_20211231_WB_M_R_w_23061202_308.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_44510_20220128_ICC_IF_w_23061202_442.webp "Anti-Glutamine synthetase antibody [GT7711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_41617_20180928_WB_B_w_23061202_816.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_41617_WB_w_23061202_387.webp "Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_44510_20231201_IHC-P_M_24010223_628.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_44825_20230609_IHC-P_multiple_M_23062718_255.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657")
![Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657](/upload/website/prouct_img/normal/GTX630657/GTX630657_44825_20230609_IHC-P_M_1_23062718_893.webp "Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657")
