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Glutamine synthetase抗体 [HL2283]

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

目录号 GTX638336

宿主

Rabbit

克隆

Monoclonal

克隆编号

HL2283

同种型

IgG

实验应用

WB, ICC/IF, IHC-P

种属反应

Human, Mouse, Rat, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

42 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length of human Glutamine synthetase.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

细胞定位

Cytoplasm, cytosol,Microsome,Mitochondrion,Cell membrane

功能与背景

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

数据库

研究领域

產品图片

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336

GTX638336 IHC-P Image

Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336

GTX638336 IHC-P Image

Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [HL2283] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638336

GTX638336 ICC/IF Image

Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at glia by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336

GTX638336 IHC-P Image

Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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