Glutamine synthetase抗体

Name: Glutamine synthetase抗体
Brand: GeneTex
SKU: GTX109121
Price: 4000.00 RMB
Rating: 5 (1 reviews)

Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121

Anti-Glutamine synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109121

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121

Anti-Glutamine synthetase antibody used in Immunoprecipitation (IP). GTX109121

Anti-Glutamine synthetase antibody used in IHC (Whole mount) (IHC-Wm). GTX109121

目录号 GTX109121

文献引用 13

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, IHC
种属反应	Human, Mouse, Rat, Zebrafish
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 Glutamine synthetase 的产品

View product citations for antibody GTX109121 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC-Wm	Assay dependent
IP	1:100-1:500
IHC	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

42 kDa. ( Note )

阳性对照

293T , DDDDK-tagged GLUL-transfected 293T

预测种属反应

Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Glutamine synthetase Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950421

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

细胞定位

Cytoplasm

功能与背景

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

数据库

Gene ID: 2752 GLUL
UniProt: P15104 GLUL

研究领域

產品图片

GTX109121 IHC-P Image

Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample:Paraffin-embedded adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121 ICC/IF Image

Glutamine synthetase antibody detects Glutamine synthetase protein at astrocytes by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109121 IHC-Fr Image

Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109121 IP Image

Immunoprecipitation of Glutamine synthetase protein from IMR32 whole cell extracts using 5 μg of Glutamine synthetase antibody (GTX109121).
Western blot analysis was performed using Glutamine synthetase antibody (GTX109121).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109121 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft , using Glutamine Synthetase (GTX109121) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109121 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.

GTX109121 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109121 WB Image

Glutamine synthetase antibody detects Glutamine synthetase protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10 % SDS-PAGE, and blotted with Glutamine synthetase antibody (GTX109121) diluted by 1:1000

GTX109121 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:20000.

GTX109121 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.

GTX109121 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:10000.

GTX109121 IHC-Wm Image

Glutamine synthetase antibody detects Glutamine synthetase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Glutamine synthetase antibody (GTX109121) dilution: 1:100.

GTX109121 WB Image

Glutamine synthetase antibody detects Glutamine synthetase protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) at a dilution of 1:10000.

GTX109121 IHC-Wm Image

Glutamine synthetase antibody detects Glutamine synthetase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Glutamine synthetase antibody (GTX109121) dilution: 1:100.

GTX109121 WB Image

The data was published in the journal Front Physiol in 2020.PMID: 32425814

GTX109121 ICC/IF Image

The data was published in the journal Sci Rep in 2019. PMID: 31616018

GTX109121 IHC-Fr Image

The data was published in the journal Front Mol Neurosci in 2019. PMID: 30930743

GTX109121 IHC-Fr Image

The data was published in the journal Front Mol Neurosci in 2019. PMID: 30930743

GTX109121 IHC-Fr Image

The data was published in the journal Front Mol Neurosci in 2019. PMID: 30930743

GTX109121 IHC-P Image

The data was published in the 2022 in Sci Rep. PMID: 35232986

GTX109121 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31616018

文献引用

Application Reference

Tabor SJ et al. Cells 2023; 12 (13) Retinal Injury Activates Complement Expression in M?ller Cells Leading to Neuroinflammation and Photoreceptor Cell Death.

Xuan DTM et al. Int J Med Sci 2023; 20 (1) : 35-49 Glutamine synthetase regulates the immune microenvironment and cancer development through the inflammatory pathway.

Ludovica Iovino et al. Acta Neuropathol 2022; 144 (1) : 81-106 Trafficking of the glutamate transporter is impaired in LRRK2-related Parkinson's disease.

实验应用 : WB

种属反应 : Human

Gallego-Dur?n R et al. Sci Rep 2022; 12 (1) : 3418 Liver injury in non-alcoholic fatty liver disease is associated with urea cycle enzyme dysregulation.

实验应用 : IHC-P

种属反应 : Human

Carmela Serpe et al. Cells 2021; 10 (8) Microglia-Derived Small Extracellular Vesicles Reduce Glioma Growth by Modifying Tumor Cell Metabolism and Enhancing Glutamate Clearance through miR-124.

实验应用 : IHC

种属反应 : Mouse

显示更多 +

我要回报文献

产品评论

Glutamine synthetase antibody 目录号 GTX109121

Rating

( Average 5 based on 1 users reviews)

实验应用

IHC (Paraffin sections)(IHC-P)

( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 25-Feb-2020

Score :

Application Tested : IHC-P

Sample Species : Hu

Sample : Brain tissue

Blocking : 25°C, 1Hr

Permeabilization /Antigen retrieval : 1%BSA, 15% goat serum/Citrate buffer

Primary Antibody : 1:500, 4°C, 18Hr

条带说明 : Red: Glutamine synthetase, Green: EAAT2, Blue: DAPI

我要评论产品

Application Reference

Tabor SJ et al. Cells 2023; 12 (13) Retinal Injury Activates Complement Expression in M?ller Cells Leading to Neuroinflammation and Photoreceptor Cell Death.

Xuan DTM et al. Int J Med Sci 2023; 20 (1):35-49 Glutamine synthetase regulates the immune microenvironment and cancer development through the inflammatory pathway.

Ludovica Iovino et al. Acta Neuropathol 2022; 144 (1):81-106 Trafficking of the glutamate transporter is impaired in LRRK2-related Parkinson's disease.

实验应用 : WB

种属反应 : Human

Gallego-Dur?n R et al. Sci Rep 2022; 12 (1):3418 Liver injury in non-alcoholic fatty liver disease is associated with urea cycle enzyme dysregulation.

实验应用 : IHC-P

种属反应 : Human

Huabo Wang et al. Cell Mol Gastroenterol Hepatol 2021; 12 (1):199-228 Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggressive Murine Hepatoblastomas.

实验应用 : WB

种属反应 : Mouse

Carmela Serpe et al. Cells 2021; 10 (8) Microglia-Derived Small Extracellular Vesicles Reduce Glioma Growth by Modifying Tumor Cell Metabolism and Enhancing Glutamate Clearance through miR-124.

实验应用 : IHC

种属反应 : Mouse

Li Q et al. J Neuroinflammation 2021; 18 (1):271 TRPV4-induced M?ller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway.

实验应用 : IHC-P

种属反应 : Rat

Schmidt A et al. J Cancer Res Clin Oncol 2021; (Epub) Hepatoblastoma: glutamine depletion hinders cell viability in the embryonal subtype but high GLUL expression is associated with better overall survival.

实验应用 : WB

种属反应 : Human

Yamada T et al. Front Physiol 2020; 11:445 Cancer Cachexia Induces Preferential Skeletal Muscle Myosin Loss When Combined With Denervation.

Mundorf A et al. Psychiatry Res 2019; 281:112544 Lithium and glutamine synthetase: Protective effects following stress.

实验应用 : IHC

种属反应 : Mouse

Pronin A et al. Front Mol Neurosci 2019; 12:36 Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury.

Nakashima K et al. Sci Rep 2019; 9 (1):14818 A spatial similarity of stereochemical environments formed by amino acid residues defines a common epitope of two non-homologous proteins.

实验应用 : ICC/IF

种属反应 : Human

Kamei Y et al. Am J Physiol Endocrinol Metab 2014; 307 (6):E485-93 FOXO1 activates glutamine synthetase gene in mouse skeletal muscles through a region downstream of 3'-UTR: possible contribution to ammonia detoxification.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

	(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
	http://www.neobioscience.com
	genetex@neobioscience.com

Glutamine synthetase抗体

目录号 GTX109121

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX109121 IHC-P Image

GTX109121 ICC/IF Image

GTX109121 WB Image

GTX109121 IHC-Fr Image

GTX109121 WB Image

GTX109121 IP Image

GTX109121 IHC-P Image

GTX109121 WB Image

GTX109121 WB Image

GTX109121 WB Image

GTX109121 WB Image

GTX109121 WB Image

GTX109121 WB Image

GTX109121 IHC-Wm Image

GTX109121 WB Image

GTX109121 IHC-Wm Image

GTX109121 WB Image

GTX109121 WB Image

GTX109121 ICC/IF Image

GTX109121 IHC-Fr Image

GTX109121 IHC-Fr Image

GTX109121 IHC-Fr Image

GTX109121 IHC-P Image

GTX109121 WB Image

文献引用

Application Reference

产品评论

Glutamine synthetase antibody 目录号 GTX109121

产品

研究

支持

公司

Follow Us

粤ICP备08007346号-9

© 1998-2022 GeneTex, Inc. 保留所有权利。

所有产品仅供研究使用 - 不应用于诊断或治疗。