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Glutathione Reductase抗体 [N2C2], Internal

KO/KD Orthogonal
Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

目录号 GTX114199

文献引用 14

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, EM

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
EM Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

56 kDa. ( Note )

阳性对照

PC-12 , Rat-2 , mouse brain , mouse eye

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Glutathione Reductase. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10620787

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glutathione-disulfide reductase , GR , HEL-75 , HEL-S-122m

细胞定位

Mitochondrion , Cytoplasm

功能与背景

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199

GTX114199 IHC-P Image

Glutathione reductase antibody [N2C2], Internal detects Glutathione reductase protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glutathione reductase antibody [N2C2], Internal (GTX114199) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody [N2C2], Internal (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199

GTX114199 IHC-P Image

Glutathione reductase antibody [N2C2], Internal detects Glutathione reductase protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glutathione reductase antibody [N2C2], Internal (GTX114199) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione reductase (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione reductase (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

KO/KD

The data was published in the journal Proteome Sci in 2014. PMID: 24405781

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

文献引用

Application Reference

M Brzozowa-Zasada et al. Int J Mol Sci 2024; 25 (2) : 1097 Glutathione Reductase Expression and Its Prognostic Significance in Colon Cancer
实验应用 : WB, IHC-P, EM
KY Lee et al. Ecotoxicol Environ Saf 2023; Downregulation of TAZ elicits a mitochondrial redox imbalance and ferroptosis in lung epithelial cells exposed to diesel exhaust particles
实验应用 : WB
Pek E et al. J Turk Ger Gynecol Assoc 2020; 21 (4) : 243-254 The immunohistochemical and histologic effects of contrast medium on uterus, fallopian tubes and ovaries, given during hysterosalpingography: rat study
Ramos H et al. Antioxidants (Basel) 2020; 9 (9)
实验应用 : WB
种属反应 : Mouse
Bai X et al. Oxid Med Cell Longev 2019; 2019 : 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
实验应用 : WB
种属反应 : Human
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Application Reference
M Brzozowa-Zasada et al. Int J Mol Sci 2024; 25 (2):1097 Glutathione Reductase Expression and Its Prognostic Significance in Colon Cancer
实验应用 : WB, IHC-P, EM
KY Lee et al. Ecotoxicol Environ Saf 2023; Downregulation of TAZ elicits a mitochondrial redox imbalance and ferroptosis in lung epithelial cells exposed to diesel exhaust particles
实验应用 : WB
Pek E et al. J Turk Ger Gynecol Assoc 2020; 21 (4):243-254 The immunohistochemical and histologic effects of contrast medium on uterus, fallopian tubes and ovaries, given during hysterosalpingography: rat study
Ramos H et al. Antioxidants (Basel) 2020; 9 (9)
实验应用 : WB
种属反应 : Mouse
Bai X et al. Oxid Med Cell Longev 2019; 2019:2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
实验应用 : WB
种属反应 : Human
Jeongsu Han et al. Oxidative Medicine and Cellular Longevity 2019; 2019:Article ID 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
实验应用 : WB
种属反应 : Human
Saudrais E et al. Am J Physiol Cell Physiol 2018; (Epub) Choroid plexus glutathione peroxidases are instrumental in protecting the brain fluid environment from hydroperoxides during postnatal development.
Maclean KN et al. FASEB J 2017; (Epub) Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.
实验应用 : WB
种属反应 : Mouse
Zimnol A et al. Am J Physiol Renal Physiol 2017;:ajprenal.00183.2017 Angiotensin II type 1a receptor-deficient mice develop angiotensin II-induced oxidative stress and DNA damage without blood pressure increase.
实验应用 : WB
种属反应 : Mouse
Wang YY et al. Head Neck 2017; (Epub) Synthetic β-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction.
实验应用 : WB
种属反应 : Human
Huang CS et al. Biochem Pharmacol 2015; 93 (3):352-61 Shikonin inhibits oxidized LDL-induced monocyte adhesion by suppressing NFκB activation via up-regulation of PI3K/Akt/Nrf2-dependent antioxidation in EA.hy926 endothelial cells.
实验应用 : WB
种属反应 : Human
Chi-Rei Wu et al. Chemico-Biological Interactions 2015; 225:40–46 Carnosic acid protects against 6-hydroxydopamine-induced neurotoxicity in in-vivo and in-vitro model of Parkinson’s disease: Involvement of antioxidative enzymes induction
实验应用 : WB
种属反应 : Rat
Chou HC et al. Proteome Sci 2014; 12 (1):2 Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma.
实验应用 : WB
种属反应 : Human
Fan CY et al. Electrophoresis 2013; 34 (24):3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
实验应用 : WB
种属反应 : Human
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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