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Glypican 1抗体 [N3C3]

KO/KD Comparison Orthogonal
Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Flow cytometry (FACS). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Immunoprecipitation (IP). GTX104557
Anti-Glypican 1 antibody [N3C3] used in ELISA (ELISA). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot and Immunoprecipitation (WB IP). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot and Immunoprecipitation (WB IP). GTX104557
Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557
Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557

目录号 GTX104557

文献引用 11
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, FACS, IP, ELISA

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Glypican 1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
ELISA 1:1000-1:10000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

62 kDa. ( Note )

阳性对照

MDA-MB-231 , MDA-MB-231 (24 μg/ml Tunicamycin for 16hr) , U87-MG membrane extracts , U87-MG conditioned medium , Mouse brain , mouse fetal brain , A431 , MCF-7 , AsPC-1

预测种属反应

Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950436

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glypican 1 , glypican

功能与背景

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557

GTX104557 IHC-P Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).
Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

Anti-Glypican 1 antibody [N3C3] used in Flow cytometry (FACS). GTX104557

GTX104557 FACS Image

Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis.
Sample: A431 cell.
Black: Unlabelled sample was used as a control.
Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:100.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Glypican 1 antibody [N3C3] used in Immunoprecipitation (IP). GTX104557

GTX104557 IP Image

Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 μg of Glypican 1 antibody [N3C3] (GTX104557).
Western blot analysis was performed using Glypican 1 antibody [N3C3] (GTX104557).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Glypican 1 antibody [N3C3] used in ELISA (ELISA). GTX104557

GTX104557 ELISA Image

An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (GTX104557) at concentration ranged from 7.5 to 240 ng/mL.

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Orthogonal

U87-MG whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Mouse tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557

GTX104557 IHC-P Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glypican 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104557

GTX104557 ICC/IF Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Orthogonal

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

A431 whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glypican 1 antibody [N3C3] used in Western Blot and Immunoprecipitation (WB IP). GTX104557

GTX104557 WB IP Image

KO/KD Orthogonal

The data was published in the journal Nat Commun in 2017. PMID: 28102194

Anti-Glypican 1 antibody [N3C3] used in Western Blot and Immunoprecipitation (WB IP). GTX104557

GTX104557 WB IP Image

The data was published in the journal Nat Commun in 2017. PMID: 28102194

Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557

GTX104557 IHC-P Image

The data was published in the journal Nat Commun in 2017. PMID: 28102194

Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557

GTX104557 IHC-P Image

The data was published in the journal Nat Commun in 2017. PMID: 28102194

文献引用

Application Reference

S Uchida et al. Neoplasia 2024; 50 : 100982 Glypican-1-targeted antibody–drug conjugate inhibits the growth of glypican-1-positive glioblastoma
Sukhneeraj P Kaur et al. Sci Rep 2021; 11 (1) : 9262 Inhibition of glypican-1 expression induces an activated fibroblast phenotype in a human bone marrow-derived stromal cell-line.
实验应用 : WB
种属反应 : Human
Munekage E et al. Neoplasia 2021; 23 (9) : 939-950 A glypican-1-targeted antibody-drug conjugate exhibits potent tumor?growth inhibition in glypican-1-positive pancreatic cancer and esophageal squamous cell carcinoma.
实验应用 : IHC-P
种属反应 : Human
Zhang J et al. Ecotoxicol Environ Saf 2021; 218 : 112257 Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.
实验应用 : WB, IHC-P
种属反应 : Mouse
Emmanouilidi A et al. Proteomics 2019; 19 (8) : e1800158 Oncogenic and Non-Malignant Pancreatic Exosome Cargo Reveal Distinct Expression of Oncogenic and Prognostic Factors Involved in Tumor Invasion and Metastasis.
实验应用 : WB
种属反应 : Human
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Glypican 1 antibody [N3C3] 目录号 GTX104557

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Date : Anonymous submitted on 05-May-2016
Score :
Application Tested : WB
Sample : MCF-7
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 : Lane1: Non-infected control. Lane2: Mock control. Lane3_GPC1-shRNA(10) lentivirus infection. Lane4: GPC1-shRNA(30) lentivirus infection.
我要评论产品
Application Reference
S Uchida et al. Neoplasia 2024; 50:100982 Glypican-1-targeted antibody–drug conjugate inhibits the growth of glypican-1-positive glioblastoma
Sukhneeraj P Kaur et al. Sci Rep 2021; 11 (1):9262 Inhibition of glypican-1 expression induces an activated fibroblast phenotype in a human bone marrow-derived stromal cell-line.
实验应用 : WB
种属反应 : Human
Munekage E et al. Neoplasia 2021; 23 (9):939-950 A glypican-1-targeted antibody-drug conjugate exhibits potent tumor?growth inhibition in glypican-1-positive pancreatic cancer and esophageal squamous cell carcinoma.
实验应用 : IHC-P
种属反应 : Human
Zhang J et al. Ecotoxicol Environ Saf 2021; 218:112257 Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.
实验应用 : WB, IHC-P
种属反应 : Mouse
Emmanouilidi A et al. Proteomics 2019; 19 (8):e1800158 Oncogenic and Non-Malignant Pancreatic Exosome Cargo Reveal Distinct Expression of Oncogenic and Prognostic Factors Involved in Tumor Invasion and Metastasis.
实验应用 : WB
种属反应 : Human
P Kim et al. Transl Psychiatry 2019; 9 (1):6 Abnormal ER quality control of neural GPIanchored proteins via dysfunction in ER export processing in the frontal cortex of elderly subjects with schizophrenia
实验应用 : WB
种属反应 : Human, Rat
Qian JY et al. Oncology Letters 2018; (Epub) Prognostic value of glypican-1 for patients with advanced pancreatic cancer following regional intra-arterial chemotherapy.
种属反应 : Human
Lu H et al. Cancer Med 2017; 6 (6):1181-1191 Elevated glypican-1 expression is associated with an unfavorable prognosis in pancreatic ductal adenocarcinoma.
Hsia K et al. Acta Biomater 2017; (Epub) Sphingosine-1-phosphate improves endothelialization with reduction of thrombosis in recellularized human umbilical vein graft by inhibiting syndecan-1 shedding in vitro.
实验应用 : ICC/IF
种属反应 : Human
Huang G et al. Nat Commun 2017; 8:14351 α3 Chains of type V collagen regulate breast tumour growth via glypican-1.
实验应用 : WB, ICC/IF, IHC-P
种属反应 : Human, Mouse
Bologna-Molina R et al. Appl Immunohistochem Mol Morphol 2015; 23 (2):153-60 Differential Expression of Glypican-1 in Ameloblastoma Variants.
实验应用 : IHC-P
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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