产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human GnRHR Extracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide encompassing a sequence within the Extracellular domain of human GnRHR. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
gonadotropin releasing hormone receptor , GNRHR1 , GRHR , HH7 , LHRHR , LRHR
细胞定位
Cell membrane; Multi-pass membrane protein
功能与背景
This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/templates/default/js/lightbox/images/loading.gif "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
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GTX639094 WB Image
Non-transfected (–) and transfected (+) unboiled PC-3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639094 WB Image
Unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX639094 IHC-P Image
GnRHR antibody [HL2638] detects GnRHR protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded PC-3 xenograft.GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:200 and competitor's antibody (19950-1-AP) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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GTX639094 IHC-P Image
GnRHR antibody [HL2638] detects GnRHR protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639094 IHC-P Image
GnRHR antibody [HL2638] detects GnRHR protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639094 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639094 IHC-P Image
GnRHR antibody [HL2638] detects GnRHR protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human PC-3 xenograft.GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639094 WB Image
Boiled and unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
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文献引用
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![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/upload/website/prouct_img/small/GTX639094/GTX639094_45299_20240202_WB_shRNA_watermark_24021917_540.webp "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/upload/website/prouct_img/small/GTX639094/GTX639094_45299_20240223_WB_Fraction_competitor_watermark_24022619_535.webp "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
![Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094](/upload/website/prouct_img/small/GTX639094/GTX639094_45299_20240301_IHC-P_competitor_24030600_229.webp "Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094")
![Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094](/upload/website/prouct_img/small/GTX639094/GTX639094_T-45187_20231103_IHC-P_multiple_R_23120519_451.webp "Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094")
![Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094](/upload/website/prouct_img/small/GTX639094/GTX639094_T-45187_20231103_IHC-P_M_23120519_824.webp "Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094")
![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/upload/website/prouct_img/small/GTX639094/GTX639094_T-45187_20231103_WB_B_23110819_886.webp "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
![Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094](/upload/website/prouct_img/small/GTX639094/GTX639094_T-45187_20231106_IHC-P_23111422_397.webp "Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094")
![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/upload/website/prouct_img/small/GTX639094/GTX639094_45299_20240126_WB_Fraction_24013018_158.webp "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/upload/website/prouct_img/normal/GTX639094/GTX639094_45299_20240202_WB_shRNA_watermark_24021917_540.webp "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/upload/website/prouct_img/normal/GTX639094/GTX639094_45299_20240223_WB_Fraction_competitor_watermark_24022619_535.webp "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
![Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094](/upload/website/prouct_img/normal/GTX639094/GTX639094_45299_20240301_IHC-P_competitor_24030600_229.webp "Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094")
![Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094](/upload/website/prouct_img/normal/GTX639094/GTX639094_T-45187_20231103_IHC-P_multiple_R_23120519_451.webp "Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094")
![Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094](/upload/website/prouct_img/normal/GTX639094/GTX639094_T-45187_20231103_IHC-P_M_23120519_824.webp "Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094")
![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/upload/website/prouct_img/normal/GTX639094/GTX639094_T-45187_20231103_WB_B_23110819_886.webp "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
![Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094](/upload/website/prouct_img/normal/GTX639094/GTX639094_T-45187_20231106_IHC-P_23111422_397.webp "Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094")
![Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094](/upload/website/prouct_img/normal/GTX639094/GTX639094_45299_20240126_WB_Fraction_24013018_158.webp "Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094")
