产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
NIH-3T3 , JC , BCL-1 , 293T , A431 , HeLa , HepG2
预测种属反应
Rat, Zebrafish, Bovine, Chicken, Japanese Medaka, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 40% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human Grp78. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10615807
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2
细胞定位
Endoplasmic reticulum lumen , Melanosome
功能与背景
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
数据库
研究领域
產品图片
![Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567](/templates/default/js/lightbox/images/loading.gif "Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567")
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GTX102567 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [C2C3], C-term (GTX102567) diluted at 1:1000.
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GTX102567 WB Image
Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Grp78 antibody (GTX102567) dilution: 1:1000
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GTX102567 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX102567 diluted at 1:2000
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文献引用
产品评论
目前没有使用 Grp78 antibody [C2C3], C-term (GTX102567) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567](/upload/website/prouct_img/small/GTX102567/GTX102567_39680_20160728_WB_shRNA_watermark_w_23060100_446.webp "Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567")
![Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567](/upload/website/prouct_img/small/GTX102567/GTX102567_39680_WB_w_23060100_387.webp "Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567")
![Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567](/upload/website/prouct_img/small/GTX102567/GTX102567_39680_WB_M_w_23060100_971.webp "Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567")
![Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567](/upload/website/prouct_img/normal/GTX102567/GTX102567_39680_20160728_WB_shRNA_watermark_w_23060100_446.webp "Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567")
![Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567](/upload/website/prouct_img/normal/GTX102567/GTX102567_39680_WB_w_23060100_387.webp "Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567")
![Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567](/upload/website/prouct_img/normal/GTX102567/GTX102567_39680_WB_M_w_23060100_971.webp "Anti-Grp78 antibody [C2C3], C-term used in Western Blot (WB). GTX102567")
