产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human HSPA5 protein, and it does not cross react with human HSPA1A, HSPA1L, HSPA2, HSPA6, HSPA8, and HSPA9 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human Grp78
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2
细胞定位
Melanosome,Cytoplasm
功能与背景
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
数据库
研究领域
產品图片
![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/templates/default/js/lightbox/images/loading.gif "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
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GTX637540 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637540 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637540 IHC-P Image
Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637540 ICC/IF Image
Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637540 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637540 IHC-P Image
Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637540 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
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产品评论
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![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/upload/website/prouct_img/small/GTX637540/GTX637540_44914_20230804_WB_shRNA_watermark_23080901_880.webp "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/upload/website/prouct_img/small/GTX637540/GTX637540_44914_20230421_WB_multiple_B_23042500_276.webp "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
![Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540](/upload/website/prouct_img/small/GTX637540/GTX637540_T-44816_20221118_IHC-P_M_22112219_111.webp "Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637540](/upload/website/prouct_img/small/GTX637540/GTX637540_T-44816_20221202_ICC_IF_22120719_777.webp "Anti-Grp78 antibody [HL1815] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/upload/website/prouct_img/small/GTX637540/GTX637540_T-44816_20221202_WB_M_R_22120719_664.webp "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
![Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540](/upload/website/prouct_img/small/GTX637540/GTX637540_T-44816_20221118_IHC-P_R_22112219_613.webp "Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/upload/website/prouct_img/small/GTX637540/GTX637540_44914_20230113_WB_TPM_watermark_23011618_484.webp "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/upload/website/prouct_img/normal/GTX637540/GTX637540_44914_20230804_WB_shRNA_watermark_23080901_880.webp "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/upload/website/prouct_img/normal/GTX637540/GTX637540_44914_20230421_WB_multiple_B_23042500_276.webp "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
![Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540](/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221118_IHC-P_M_22112219_111.webp "Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637540](/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221202_ICC_IF_22120719_777.webp "Anti-Grp78 antibody [HL1815] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221202_WB_M_R_22120719_664.webp "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
![Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540](/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221118_IHC-P_R_22112219_613.webp "Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540")
![Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540](/upload/website/prouct_img/normal/GTX637540/GTX637540_44914_20230113_WB_TPM_watermark_23011618_484.webp "Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540")
