产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Rat heart , Rat Lung , Rat Liver , Rat kidney , NIH-3T-3 , JC , BCL-1 , C2C12 , Raw264.7 , 293T , A431 , HeLa , HepG2
产品说明
Knockdown/Knockout validation was supported by customer review data.
预测种属反应
Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.14 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Grp78. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885657
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heat shock protein 5 , AL022860 , AU019543 , Bip , D2Wsu141e , D2Wsu17e , Grp78 , Hsce70 , SEZ-7 , Sez7 , baffled , mBiP
细胞定位
Endoplasmic reticulum lumen , Melanosome
功能与背景
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
数据库
研究领域
產品图片
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GTX127934 ICC/IF Image
Hspa5 antibody detects Hspa5 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hspa5 protein stained by Hspa5 antibody (GTX127934) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX127934 IHC-P Image
Hspa5 antibody detects Hspa5 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Hspa5 antibody (GTX127934) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127934 IHC-P Image
Hspa5 antibody detects Hspa5 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Hspa5 antibody (GTX127934) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX127934 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T-3
B: JC
C: BCL-1
D: C2C12
E: Raw264.7
7.5% SDS PAGE
GTX127934 diluted at 1:10000
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GTX127934 IHC-P Image
Hspa5 antibody detects Hspa5 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hspa5 antibody (GTX127934) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX127934 WB Image
Hspa5 antibody detects Hspa5 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody (GTX127934) diluted at a dilution of 1:10000.
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GTX127934 WB Image
Hspa5 antibody detects Hspa5 protein by Western blot analysis.
A. 50 μg Rat heart lysate/extract
B. 50 μg Rat Lung lysate/extract
C. 50 μg Rat Liver lysate/extract
D. 50 μg Rat kidney lysate/extract
7.5 % SDS-PAGE
Hspa5 antibody (GTX127934) dilution: 1:10000
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GTX127934 WB Image
The data was published in the 2018 in Sci Rep. PMID: 29891991
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GTX127934 WB Image
The data was published in the 2019 in J Clin Biochem Nutr. PMID: 30705510
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GTX127934 WB Image
The data was published in the 2019 in J Clin Biochem Nutr. PMID: 30705510
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文献引用
产品评论
Grp78 antibody
目录号 GTX127934
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Rating
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( Average 4 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 05-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
SAS cells
Amount used :
20μg
Blocking :
5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
