GeneTex中国官方网站
  • China

Grp78抗体

Orthogonal
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48633
Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633

目录号 GTX48633

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, FACS

种属反应

 Human, Mouse, Rat, Zebrafish, Sheep, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Primate
产品包装
50 μl (¥5000)
查看所有 Grp78 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 0.5 μg/ml
ICC/IF 1:50
FACS 1:150
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

72 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]

纯化方式

Purified by antigen-affinity chromatography

偶联

Unconjugated

RRID

AB_10619986

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

细胞定位

Cytoplasm

功能与背景

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

数据库

研究领域

產品图片

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48633

GTX48633 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48633 Grp78 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml

Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633

GTX48633 FACS Image

FACS (Intracellular staining) analysis of Jurkat cells using GTX48633 Grp78 antibody.
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa, A431, NIH-3T3, PC12 cell lysate using GTX48633 Grp78 antibody.
Dilution : 1 μg/ml

Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633

GTX48633 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX48633 Grp78 antibody.
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa cell lysate using GTX48633 Grp78 antibody.

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa cell lysate using GTX48633 Grp78 antibody.

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

Orthogonal

The data was published in the journal Elife in 2016.PMID: 27669143

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

Orthogonal

The data was published in the journal Elife in 2016. PMID: 27669143

文献引用

Application Reference

Wu Z et al. Ecotoxicol Environ Saf 2021; 221 : 112438 Inhibition of endoplasmic reticulum stress-related autophagy attenuates MCLR-induced apoptosis in zebrafish testis and mouse TM4 cells.
实验应用 : WB
种属反应 : Zebrafish
Sujuan Zhao et al. Environmental Pollution 2019; (Epub) Microcystin-LR exposure decreased the fetal weight of mice by disturbance of placental development and ROS-mediated endoplasmic reticulum stress in the placenta.
实验应用 : WB
Vanhoutte D et al. Elife 2016; 5 (Epub) Thrombospondin expression in myofibers stabilizes muscle membranes.
实验应用 : WB
种属反应 : Drosophila
我要回报文献

产品评论

目前没有使用 Grp78 antibody (GTX48633) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5000
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com