Name: HA tag抗体 [GT423]
Brand: GeneTex
SKU: GTX628489
Price: 4000.00 RMB
Rating: 3 (1 reviews)

HA tag抗体 [GT423]

目录号 GTX628489

1 引用文献 13

目录号 GTX628489

GTX628489 WB Image

HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 μg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX628489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX628489 IP Image

HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments.
IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells
A. Control with 2 μg of preimmune mouse IgG
B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] (GTX628489)
5% SDS-PAGE
The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody (GTX127309) diluted at 1:1000.

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GTX628489 ICC/IF Image

HA tag antibody [GT423] detects HA protein by immunofluorescent analysis.
Sample: HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA stained by HA tag antibody [GT423] (GTX628489) diluted at 1:2000.
Blue: Hoechst 33342 staining.

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GTX628489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000 and competitor's antibody (sc-7392) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

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