HDAC1抗体

Name: HDAC1抗体
Brand: GeneTex
SKU: GTX100513
Price: 4000.00 RMB
Rating: 4 (1 reviews)

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513

Anti-HDAC1 antibody used in IHC (Whole mount) (IHC-Wm). GTX100513

Anti-HDAC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100513

目录号 GTX100513

文献引用 33

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ChIP assay
种属反应	Human, Mouse, Rat, Zebrafish
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX100513 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
IHC-Wm	Assay dependent
IP	1:100-1:500
ChIP assay	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

55 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , Rat-2 , NIH3T3 , DDDDK-tagged HDAC1-transfected 293T

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240929

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1

细胞定位

Nucleus

功能与背景

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]

数据库

Gene ID: 3065 HDAC1
UniProt: Q13547 HDAC1

研究领域

產品图片

GTX100513 WB Image

Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100513 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100513 WB Image

HDAC1 antibody detects HDAC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100513 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (GTX100513) at 1:300 dilution.

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
HDAC1 antibody (GTX100513) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100513 IP Image

HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC1 protein by 2.5 μg of HDAC1 antibody (GTX100513) 10% SDS-PAGE The immunoprecipitated HDAC1 protein was detected by HDAC1 antibody (GTX100513) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100513 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: HDAC1 antibody (GTX100513) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HDAC1 antibody (GTX100513) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100513 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1(GTX100513) antibody at 1:200 dilution.

GTX100513 ICC/IF Image

HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC1 stained by HDAC1 antibody (GTX100513) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100513 ChIP assay Image

HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC1 antibody (GTX100513) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

GTX100513 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100513 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX100513 diluted at 1:1000

GTX100513 IHC-Wm Image

HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
HDAC1 antibody (GTX100513) dilution: 1:100.

GTX100513 IHC-Fr Image

The data was published in the journal PLoS Biol in 2015.PMID: 26406915

GTX100513 WB Image

The data was published in the journal Nat Commun in 2018.PMID: 30242288

GTX100513 WB Image

The data was published in the journal Nat Commun in 2018.PMID: 30242288

GTX100513 WB Image

The data was published in the journal Front Pharmacol in 2016.PMID: 27065869

GTX100513 IHC-Fr Image

The data was published in the journal J Cell Mol Med in 2020.PMID: 32227584

GTX100513 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28139683

GTX100513 WB Image

The data was published in the journal Int J Mol Sci in 2019.PMID: 30934807

GTX100513 WB Image

The data was published in the journal PLoS Biol in 2015.PMID: 26406915

GTX100513 IHC-Fr Image

The data was published in the journal Nat Commun in 2017. PMID: 28139683

文献引用

Application Reference

Xu Z et al. Cell Death Discov 2022; 8 (1) : 474 Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer.

Oliver Clau? et al. Pharmaceuticals (Basel) 2022; 15 (3) Development and Biological Evaluation of the First Highly Potent and Specific Benzamide-Based Radiotracer [¹⁸F]BA3 for Imaging of Histone Deacetylases 1 and 2 in Brain.

实验应用 : ICC/IF

种属反应 : Human

Lin HY et al. Biomed Pharmacother 2022; 145 : 112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.

实验应用 : WB

种属反应 : Human

Hang Yuan et al. Int J Mol Sci 2021; 22 (16) PPARγ-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts.

实验应用 : WB

种属反应 : Human

Abdollahi S et al. Biomark Res 2021; 9 (1) : 74 Deciphering genes associated with diffuse large B-cell lymphoma with lymphomatous effusions: A mutational accumulation scoring approach.

实验应用 : IHC-P

种属反应 : Human

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我要回报文献

产品评论

HDAC1 antibody 目录号 GTX100513

Rating

( Average 4 based on 1 users reviews)

实验应用

Western Blot(WB)

( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 30-Apr-2013

Score :

Application Tested : WB

Sample Species : Ms

Sample : E8.5 mouse embryo tissue

Amount used : 40μg

Blocking : 4°C, 2Hr

Primary Antibody : 1:1000, 4°C, 16Hr

我要评论产品

Application Reference

Xu Z et al. Cell Death Discov 2022; 8 (1):474 Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer.

实验应用 : ICC/IF

种属反应 : Human

Lin HY et al. Biomed Pharmacother 2022; 145:112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.

实验应用 : WB

种属反应 : Human

Hang Yuan et al. Int J Mol Sci 2021; 22 (16) PPARγ-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts.

实验应用 : WB

种属反应 : Human

Abdollahi S et al. Biomark Res 2021; 9 (1):74 Deciphering genes associated with diffuse large B-cell lymphoma with lymphomatous effusions: A mutational accumulation scoring approach.

实验应用 : IHC-P

种属反应 : Human

Chen K et al. Cancer Commun (Lond) 2021; 41 (4):316-332 Junction plakoglobin regulates and destabilizes HIF2α to inhibit tumorigenesis of renal cell carcinoma.

实验应用 : WB

种属反应 : Human

Lin YC et al. Int J Mol Sci 2020; 21 (12) Nifedipine Exacerbates Lipogenesis in the Kidney via KIM-1, CD36, and SREBP Upregulation: Implications from an Animal Model for Human Study.

Ochiai H et al. Sci Adv 2020; 6 (25):eaaz6699 Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.

Hu TM et al. Brain Sci 2020; 10 (8)

实验应用 : WB

种属反应 : Human

Deng M et al. J Cell Mol Med 2020; 24 (9):5249-5259 Overexpression of peptidase inhibitor 16 attenuates angiotensin II-induced cardiac fibrosis via regulating HDAC1 of cardiac fibroblasts.

实验应用 : IHC-Fr

种属反应 : Mouse, Rat

Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.

实验应用 : WB

种属反应 : Human

Gao Z et al. RNA Biol 2019; 16 (7):940-949 A novel human lncRNA SANT1 cis-regulates the expression of SLC47A2 by altering SFPQ/E2F1/HDAC1 binding to the promoter region in renal cell carcinoma.

Wu TH et al. Int J Mol Sci 2019; 20 (23)

实验应用 : WB

种属反应 : Human

Lin YC et al. Int J Mol Sci 2019; 20 (7) Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway.

2019; (Epub) Loss of ARID1A induces a stemness gene ALDH1A1 expression with histone acetylation in the malignant subtype of cholangiocarcinoma.

实验应用 : IP

Yokoji-Takeuchi M et al. Arch Oral Biol 2019; 110:104602 A bacterial metabolite induces Nrf2-mediated anti-oxidative responses in gingival epithelial cells by activating the MAPK signaling pathway.

实验应用 : WB

种属反应 : Human

Yuanyuan Ye et al. International Journal of Oncology 2019;:1019-1032 SIP1 serves a role in HBx?induced liver cancer growth and metastasis.

实验应用 : WB

种属反应 : Human

Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.

实验应用 : WB

种属反应 : Human

Jiao D et al. Oncogene 2019; (Epub) UHRF1 promotes renal cell carcinoma progression through epigenetic regulation of TXNIP.

实验应用 : ChIP assay

种属反应 : Human

Liu Y et al. J Biol Chem 2018; 293 (36):14012-14021 Kr?ppel-like Factor 10 (KLF10) Is Transactivated by the Transcription Factor C/EBPβ and Involved in Early 3T3-L1 Preadipocyte Differentiation.

Lee HS et al. Nat Commun 2018; 9 (1):3848 The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation.

实验应用 : WB

种属反应 : Human

Huang D et al. Nat Immunol 2018; (Epub) NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

实验应用 : ChIP assay

种属反应 : Human

Sulijaya B et al. J Periodontal Res 2018; (Epub) The anti-inflammatory effect of 10-oxo-trans-11-octadecenoic acid (KetoC) on RAW 264.7 cells stimulated with Porphyromonas gingivalis lipopolysaccharide.

实验应用 : WB

种属反应 : Mouse

Brugger V et al. Nat Commun 2017; 8:14272 Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.

Lee JY et al. Front Pharmacol 2016; 7:81 Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.

实验应用 : WB

种属反应 : Human

Tsai HD et al. Neuromolecular Med 2016; (Epub) Clinacanthus nutans Protects Cortical Neurons Against Hypoxia-Induced Toxicity by Downregulating HDAC1/6.

实验应用 : WB

种属反应 : Mouse

Baek MH et al. Anticancer Res 2016; 36 (5):2527-34 Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma.

实验应用 : IHC-P

Brugger V et al. PLoS Biol 2015; 13 (9):e1002258 HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.

实验应用 : WB, ICC/IF

种属反应 : Mouse

Lokireddy S et al. Proc Natl Acad Sci U S A 2015; 112 (52):E7176-85 cAMP-induced phosphorylation of 26S proteasomes on Rpn6/PSMD11 enhances their activity and the degradation of misfolded proteins.

Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.

实验应用 : WB

种属反应 : Human

Kumar S et al. Development 2014; 141 (15):391724 Retinoic acid controls body axis extension by directly repressing Fgf8 transcription.

实验应用 : ChIP assay

种属反应 : Mouse

Chang JF et al. Toxicol Sci 2014; (139):396-406 nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.

实验应用 : WB

种属反应 : Human

Yoon JH et al. Biochem Pharmacol 2012; 83 (6):747-57 Role of autophagy in chemoresistance: regulation of the ATM-mediated DNA-damage signaling pathway through activation of DNA-PKcs and PARP-1.

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产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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HDAC1抗体

目录号 GTX100513

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX100513 WB Image

GTX100513 WB Image

GTX100513 WB Image

GTX100513 IHC-P Image

GTX100513 WB Image

GTX100513 IHC-P Image

GTX100513 IHC-P Image

GTX100513 IP Image

GTX100513 WB Image

GTX100513 WB Image

GTX100513 IHC-P Image

GTX100513 IHC-P Image

GTX100513 ICC/IF Image

GTX100513 ICC/IF Image

GTX100513 IHC-P Image

GTX100513 ChIP assay Image

GTX100513 WB Image

GTX100513 WB Image

GTX100513 IHC-Wm Image

GTX100513 IHC-Fr Image

GTX100513 WB Image

GTX100513 WB Image

GTX100513 WB Image

GTX100513 IHC-Fr Image

GTX100513 WB Image

GTX100513 WB Image

GTX100513 WB Image

GTX100513 IHC-Fr Image

文献引用

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HDAC1 antibody 目录号 GTX100513

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