HDAC2抗体

Name: HDAC2抗体
Brand: GeneTex
SKU: GTX109642
Price: 4000.00 RMB
Rating: 5 (1 reviews)

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX109642

Anti-HDAC2 antibody used in ChIP assay (ChIP assay). GTX109642

Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109642

Anti-HDAC2 antibody used in Immunoprecipitation (IP). GTX109642

Anti-HDAC2 antibody used in Western Blot (WB). GTX109642

目录号 GTX109642

文献引用 23

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP, ChIP assay
种属反应	Human, Mouse, Rat, Xenopus tropicalis
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX109642 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

55 kDa. ( Note )

阳性对照

C2C12 , Rat2

产品说明

Knockdown/Knockout validation was supported by customer review data.

预测种属反应

Bovine, Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950481

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1

细胞定位

Nucleus

功能与背景

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq]

数据库

Gene ID: 3066 HDAC2
UniProt: Q92769 HDAC2

研究领域

產品图片

GTX109642 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using HDAC2(GTX109642) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109642 ChIP assay Image

HDAC2 antibody immunoprecipitates HDAC2 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract
A. 5 μg preimmune rabbit IgG
B. 5 μg of HDAC2 antibody (GTX109642)
The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.

GTX109642 ICC/IF Image

HDAC2 antibody detects HDAC2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC2 stained by HDAC2 antibody (GTX109642) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109642 IP Image

HDAC2 antibody immunoprecipitates HDAC2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC2 protein by 2 μg of HDAC2 antibody (GTX109642) 7.5% SDS-PAGE The immunoprecipitated HDAC2 protein was detected by HDAC2 antibody (GTX109642) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109642 ICC/IF Image

GTX109642 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC2 antibody (GTX109642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109642 WB Image

HDAC2 antibody detects HDAC2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
HDAC2 antibody (GTX109642) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109642 WB Image

HDAC2 antibody detects HDAC2 protein by western blot analysis.
A. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
HDAC2 antibody (GTX109642) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109642 WB Image

The data was published in the journal Front Pharmacol in 2017.PMID: 29326587

GTX109642 WB Image

The data was published in the journal Front Pharmacol in 2016.PMID: 27065869

GTX109642 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35163312

文献引用

Application Reference

Islam A et al. Nucleic Acids Res 2024; 52 (5) : 2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.

N Tapryal et al. Nat Commun 2023; 14 (1) : 8169 The DNA glycosylase NEIL2 is protective during SARS-CoV-2 infection

实验应用 : WB

Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.

Zhou JJ et al. Elife 2023; 12 Histone deacetylase 1 maintains lineage integrity through histone acetylome refinement during early embryogenesis.

实验应用 : WB

种属反应 : Xenopus tropicalis

Duan Y et al. mBio 2022; : e0328721 Novel Function of Avian p53 in Binding to ALV-J LTR Contributes to Its Antiviral Roles.

实验应用 : ICC/IF

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产品评论

HDAC2 antibody 目录号 GTX109642

Rating

( Average 5 based on 1 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 21-May-2015

Score :

Application Tested : WB

Sample Species : Ms

Sample : NB2a cells

Amount used : 5μg

Blocking : 10% skim milk, 25°C, 1Hr

Primary Antibody : 1:200, 25°C, 1Hr

条带说明 : Lane 1:NB2a cells; Lane 2:knockout HDAC2 in NB2a cells; Lane 3:knockout HDAC1 in NB2a cells

我要评论产品

Application Reference

Islam A et al. Nucleic Acids Res 2024; 52 (5):2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.

N Tapryal et al. Nat Commun 2023; 14 (1):8169 The DNA glycosylase NEIL2 is protective during SARS-CoV-2 infection

实验应用 : WB

Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.

Zhou JJ et al. Elife 2023; 12 Histone deacetylase 1 maintains lineage integrity through histone acetylome refinement during early embryogenesis.

实验应用 : WB

种属反应 : Xenopus tropicalis

Yang HH et al. Int J Mol Sci 2022; 23 (3) Anti-Excitotoxic Effects of N-Butylidenephthalide Revealed by Chemically Insulted Purkinje Progenitor Cells Derived from SCA3 iPSCs.

实验应用 : WB

种属反应 : Human

Duan Y et al. mBio 2022;:e0328721 Novel Function of Avian p53 in Binding to ALV-J LTR Contributes to Its Antiviral Roles.

实验应用 : ICC/IF

Lin HY et al. Biomed Pharmacother 2022; 145:112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.

实验应用 : WB

种属反应 : Human

Nisha Tapryal et al. J Biol Chem 2021; 296:100723 Intrapulmonary administration of purified NEIL2 abrogates NF-κB-mediated inflammation.

实验应用 : WB

种属反应 : Mouse

Sayed IM et al. Cells 2020; 9 (9) The DNA Glycosylase NEIL2 Suppresses Fusobacterium-Infection-Induced Inflammation and DNA Damage in Colonic Epithelial Cells.

实验应用 : WB

种属反应 : Mouse

Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.

实验应用 : WB

种属反应 : Human

Thang SK et al. J Agric Food Chem 2019; 67 (40):11119-11128 Xanthohumol Suppresses NPC1L1 Gene Expression through Downregulation of HNF-4α and Inhibits Cholesterol Uptake in Caco-2 Cells.

实验应用 : WB

种属反应 : Human

Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.

实验应用 : WB

种属反应 : Human

Chang HY et al. Molecules 2019; 24 (3) The Cholesterol-Modulating Effect of Methanol Extract of Pigeon Pea (Cajanus cajan (L.) Millsp.) Leaves on Regulating LDLR and PCSK9 Expression in HepG2 Cells.

Huang D et al. Nat Immunol 2018; (Epub) NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

实验应用 : ChIP assay

种属反应 : Human

Gao WY et al. J Agric Food Chem 2018; 66 (24):6083-6093 Pinostrobin Inhibits Proprotein Convertase Subtilisin/Kexin-type 9 (PCSK9) Gene Expression through the Modulation of FoxO3a Protein in HepG2 Cells.

实验应用 : WB

种属反应 : Human

Cencioni C et al. Nat Commun 2018; 9 (1):1281 Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells.

实验应用 : IP

种属反应 : Mouse

Huang WT et al. Front Pharmacol 2017; 8:902 HDAC2 and HDAC5 Up-Regulations Modulate Survivin and miR-125a-5p Expressions and Promote Hormone Therapy Resistance in Estrogen Receptor Positive Breast Cancer Cells.

Chen SF et al. J Agric Food Chem 2017; (Epub) Xanthohumol suppresses Mylip/Idol gene expression and modulates LDLR abundance and activity in HepG2 cells.

实验应用 : WB

种属反应 : Human

Tanegashima K et al. Genes Cells 2017; 22 (1):71-83 Epigenetic regulation of the glucose transporter gene Slc2a1 by β-hydroxybutyrate underlies preferential glucose supply to the brain of fasted mice.

实验应用 : WB

种属反应 : Mouse

Lee JY et al. Front Pharmacol 2016; 7:81 Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.

实验应用 : WB

种属反应 : Human

Chen HC et al. PLoS One 2016; 11 (9):e0162414 Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.

实验应用 : WB

种属反应 : Human

Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.

实验应用 : WB

种属反应 : Human

Chen PY et al. PLoS One 2013; 8 (8):e71282 Down-regulation of the oncogene PTTG1 via the KLF6 tumor suppressor during induction of myeloid differentiation.

实验应用 : WB

种属反应 : Human

SDS
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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HDAC2抗体

目录号 GTX109642

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品说明

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX109642 IHC-P Image

GTX109642 ChIP assay Image

GTX109642 ICC/IF Image

GTX109642 IP Image

GTX109642 ICC/IF Image

GTX109642 WB Image

GTX109642 WB Image

GTX109642 WB Image

GTX109642 WB Image

GTX109642 WB Image

GTX109642 WB Image

文献引用

Application Reference

产品评论

HDAC2 antibody 目录号 GTX109642

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