产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide of human HES1.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
hes family bHLH transcription factor 1 , HES-1 , HHL , HRY , bHLHb39
细胞定位
Nucleus
功能与背景
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202](/templates/default/js/lightbox/images/loading.gif "Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202")
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GTX638202 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638202 ICC/IF Image
HES1 antibody [HL2198] detects HES1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HES1 stained by HES1 antibody [HL2198] (GTX638202) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX638202 WB Image
MCF-7 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638202 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202](/upload/website/prouct_img/small/GTX638202/GTX638202_45026_20230428_WB_TPM_watermark_23050223_275.webp "Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202")
![Anti-HES1 antibody [HL2198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638202](/upload/website/prouct_img/small/GTX638202/GTX638202_T-44942_20230428_ICC_IF_23050918_222.webp "Anti-HES1 antibody [HL2198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638202")
![Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202](/upload/website/prouct_img/small/GTX638202/GTX638202_T-44942_20230224_WB_Fraction_23030219_736.webp "Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202")
![Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202](/upload/website/prouct_img/small/GTX638202/GTX638202_45026_20230526_WB_23053001_290.webp "Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202")
![Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202](/upload/website/prouct_img/normal/GTX638202/GTX638202_45026_20230428_WB_TPM_watermark_23050223_275.webp "Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202")
![Anti-HES1 antibody [HL2198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638202](/upload/website/prouct_img/normal/GTX638202/GTX638202_T-44942_20230428_ICC_IF_23050918_222.webp "Anti-HES1 antibody [HL2198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638202")
![Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202](/upload/website/prouct_img/normal/GTX638202/GTX638202_T-44942_20230224_WB_Fraction_23030219_736.webp "Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202")
![Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202](/upload/website/prouct_img/normal/GTX638202/GTX638202_45026_20230526_WB_23053001_290.webp "Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202")
