产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:10000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human HEY1.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
hes related family bHLH transcription factor with YRPW motif 1 , BHLHb31 , CHF2 , HERP2 , HESR1 , HRT-1 , NERP2 , OAF1 , hHRT1
细胞定位
Nucleus
功能与背景
This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX638573 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638573 WB Image
Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638573 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638573 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638573 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638573 IHC-P Image
HEY1 antibody [HL2367] detects HEY1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.HEY1 stained by HEY1 antibody [HL2367] (GTX638573) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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