产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human HHIP
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
hedgehog interacting protein , HIP
细胞定位
Cell membrane,Secreted,Cytoplasm
功能与背景
This gene encodes a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.[provided by RefSeq, Feb 2011]
数据库
研究领域
產品图片
![Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862](/templates/default/js/lightbox/images/loading.gif "Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862")
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GTX637862 IHC-P Image
HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.HHIP stained by HHIP antibody [HL1969] (GTX637862) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637862 IHC-P Image
HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.HHIP stained by HHIP antibody [HL1969] (GTX637862) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637862 IHC-P Image
HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis.Sample: Paraffin-embedded human hepatocellular carcinoma.HHIP stained by HHIP antibody [HL1969] (GTX637862) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637862 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1969] (GTX637862) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637862 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1969] (GTX637862) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
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产品评论
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![Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862](/upload/website/prouct_img/small/GTX637862/GTX637862_T-44858_20221111_IHC-P_M_1_22122018_387.webp "Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862")
![Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862](/upload/website/prouct_img/small/GTX637862/GTX637862_T-44858_20221111_IHC-P_M_22122018_596.webp "Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862")
![Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862](/upload/website/prouct_img/small/GTX637862/GTX637862_T-44858_20221111_IHC-P_22122018_460.webp "Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862")
![Anti-HHIP antibody [HL1969] used in Western Blot (WB). GTX637862](/upload/website/prouct_img/small/GTX637862/GTX637862_44935_20230714_WB_B_23071822_359.webp "Anti-HHIP antibody [HL1969] used in Western Blot (WB). GTX637862")
![Anti-HHIP antibody [HL1969] used in Western Blot (WB). GTX637862](/upload/website/prouct_img/small/GTX637862/GTX637862_44935_20230203_WB_TPM_watermark_23020621_182.webp "Anti-HHIP antibody [HL1969] used in Western Blot (WB). GTX637862")
![Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862](/upload/website/prouct_img/normal/GTX637862/GTX637862_T-44858_20221111_IHC-P_M_1_22122018_387.webp "Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862")
![Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862](/upload/website/prouct_img/normal/GTX637862/GTX637862_T-44858_20221111_IHC-P_M_22122018_596.webp "Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862")
![Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862](/upload/website/prouct_img/normal/GTX637862/GTX637862_T-44858_20221111_IHC-P_22122018_460.webp "Anti-HHIP antibody [HL1969] used in IHC (Paraffin sections) (IHC-P). GTX637862")
![Anti-HHIP antibody [HL1969] used in Western Blot (WB). GTX637862](/upload/website/prouct_img/normal/GTX637862/GTX637862_44935_20230714_WB_B_23071822_359.webp "Anti-HHIP antibody [HL1969] used in Western Blot (WB). GTX637862")
![Anti-HHIP antibody [HL1969] used in Western Blot (WB). GTX637862](/upload/website/prouct_img/normal/GTX637862/GTX637862_44935_20230203_WB_TPM_watermark_23020621_182.webp "Anti-HHIP antibody [HL1969] used in Western Blot (WB). GTX637862")
