产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2 , HepG2 ( 1% O2 treatment for 24 hr)
预测种属反应
Rat, Rabbit, Sheep, Bovine, Guinea pig, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 beta. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2861418
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
aryl hydrocarbon receptor nuclear translocator , HIF-1-beta , HIF-1beta , HIF1-beta , HIF1B , HIF1BETA , TANGO , bHLHe2
细胞定位
Nucleus
功能与背景
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX128795 IHC-P Image
HIF1 beta antibody detects HIF1 beta protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HIF1 beta antibody (GTX128795) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX128795 WB Image
HIF1 beta antibody detects HIF1 beta protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract ( 1% O2 treatment for 24 hr)
7.5 % SDS-PAGE
HIF1 beta antibody (GTX128795) dilution: 1:1000
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GTX128795 WB Image
HIF1 beta antibody detects HIF1 beta protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
HIF1 beta antibody (GTX128795) dilution: 1:1000
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GTX128795 ICC/IF Image
HIF1 beta antibody detects HIF1 beta protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 500μM CoCl2 for 24hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 beta protein stained by HIF1 beta antibody (GTX128795) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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文献引用
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产品评论
HIF1 beta antibody
目录号 GTX128795
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Rating
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( Average 4 based on 2 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4 based on 2 users reviews)
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Date :
Anonymous submitted on 08-Sep-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
skin fibroblast tissue
Amount used :
5μg
Blocking :
1% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 19-Aug-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
B16 melanoma cells
Amount used :
20μg
Blocking :
5% skim milk in PBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
条带说明 :
HIF1 beta antibody detects HIF1 beta protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment ) B16 melanoma cell extracts (20 μg) were separated by SDS-PAGE.
