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HIF2 alpha抗体 [GT125]

Orthogonal
Anti-HIF2 alpha antibody [GT125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632015
Anti-HIF2 alpha antibody [GT125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632015
Anti-HIF2 alpha antibody [GT125] used in Immunoprecipitation (IP). GTX632015
Anti-HIF2 alpha antibody [GT125] used in Western Blot (WB). GTX632015
Anti-HIF2 alpha antibody [GT125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632015
Anti-HIF2 alpha antibody [GT125] used in Western Blot (WB). GTX632015

目录号 GTX632015

文献引用 3
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT125

同种型

 IgG1

实验应用

 WB, ICC/IF, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 HIF2 alpha 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:700
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

96 kDa. ( Note )

阳性对照

HepG2 (1% O2 treatment for 24hr)

预测种属反应

Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888277

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73

细胞定位

Nucleus

功能与背景

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

数据库

研究领域

產品图片

Anti-HIF2 alpha antibody [GT125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632015

GTX632015 ICC/IF Image

HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Red: Actin filaments were labeled with Phalloidin.

Anti-HIF2 alpha antibody [GT125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632015

GTX632015 ICC/IF Image

HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Blue: Hoechst 33342 staining.
Anti-HIF2 alpha antibody [GT125] used in Immunoprecipitation (IP). GTX632015

GTX632015 IP Image

Immunoprecipitation of HIF2 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF2 alpha antibody [GT125] (GTX632015).
Western blot analysis was performed using HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-HIF2 alpha antibody [GT125] used in Western Blot (WB). GTX632015

GTX632015 WB Image

Orthogonal

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX632015) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HIF2 alpha antibody [GT125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632015

GTX632015 ICC/IF Image

HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Samples: PC-12 cells mock (left) and treated with 500 μM CoCl2 for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-HIF2 alpha antibody [GT125] used in Western Blot (WB). GTX632015

GTX632015 WB Image

Orthogonal

Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Kundu A et al. J Exp Med 2020; 217 (6) PRDM16 suppresses HIF-targeted gene expression in kidney cancer.
实验应用 : WB
种属反应 : Human
Chen L et al. Sci Rep 2016; 6 : 32582 Physapubescin selectively induces apoptosis in VHL-null renal cell carcinoma cells through down-regulation of HIF-2α and inhibits tumor growth.
实验应用 : WB
种属反应 : Human
Lin CS et al. Int J Mol Sci 2016; 17 (6) Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
实验应用 : WB
种属反应 : Human
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产品评论

HIF2 alpha antibody [GT125] 目录号 GTX632015

Rating
( Average 5 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-HIF2 alpha antibody [GT125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632015
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 100°C, 30minHr
Primary Antibody : 1:400, 25°C, 0.5Hr
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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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