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HIF2 alpha抗体

Anti-HIF2 alpha antibody used in Western Blot (WB). GTX128793
Anti-HIF2 alpha antibody used in Immunoprecipitation (IP). GTX128793

目录号 GTX128793

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, IP

种属反应

Human
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

96 kDa. ( Note )

阳性对照

HepG2 (1% O2 treatment for 24hr)

预测种属反应

Mouse, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885814

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73

细胞定位

Nucleus

功能与背景

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-HIF2 alpha antibody used in Western Blot (WB). GTX128793

GTX128793 WB Image

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX128793) diluted by 1:500.

Anti-HIF2 alpha antibody used in Immunoprecipitation (IP). GTX128793

GTX128793 IP Image

Immunoprecipitation of HIF2 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF2 alpha antibody (GTX128793).
Western blot analysis was performed using HIF2 alpha antibody (GTX128793).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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