产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human HMGB1 protein, and it does not cross react with human HMGB2 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1
细胞定位
Nucleus,Cytoplasm,Secreted,Cell membrane,Endosome
功能与背景
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
数据库
研究领域
產品图片
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/templates/default/js/lightbox/images/loading.gif "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
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GTX637963 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637963 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637963 ICC/IF Image
HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:250.Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
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GTX637963 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637963 ELISA Image
Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, human HMGB1 protein (1000-15.63 ng/mL). Coated protein was probed with HMGB1 antibody [HL2059] (GTX637963) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX637963 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637963 IHC-P Image
HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637963 IHC-P Image
HMGB1 antibody [HL2059] detects HMGB1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637963 ICC/IF Image
HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX637963 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_44935_20230210_WB_shRNA_watermark_23021401_300.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20221209_WB_M_R_22121123_430.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20230324_ICC_IF_R_23041023_472.webp "Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_44935_20230901_WB_multiple_B_23090619_386.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20220113_ELISA_Indirect_23011618_221.webp "Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_44935_20230203_WB_23020621_399.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20221230_IHC-P_multiple_M_23020621_380.webp "Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_44935_20230721_IHC-P_Cat_23073119_408.webp "Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_T-44886_20230203_ICC_IF_23021401_804.webp "Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/small/GTX637963/GTX637963_44935_20230616_WB_D_23062019_603.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230210_WB_shRNA_watermark_23021401_300.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20221209_WB_M_R_22121123_430.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20230324_ICC_IF_R_23041023_472.webp "Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230901_WB_multiple_B_23090619_386.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20220113_ELISA_Indirect_23011618_221.webp "Anti-HMGB1 antibody [HL2059] used in ELISA (ELISA). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230203_WB_23020621_399.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20221230_IHC-P_multiple_M_23020621_380.webp "Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230721_IHC-P_Cat_23073119_408.webp "Anti-HMGB1 antibody [HL2059] used in IHC (Paraffin sections) (IHC-P). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20230203_ICC_IF_23021401_804.webp "Anti-HMGB1 antibody [HL2059] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637963")
![Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963](/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230616_WB_D_23062019_603.webp "Anti-HMGB1 antibody [HL2059] used in Western Blot (WB). GTX637963")
