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HPRT抗体 [HL2015]

Recombinant antibody KO/KD Orthogonal
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637916
Anti-HPRT antibody [HL2015] used in IHC (Paraffin sections) (IHC-P). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in IHC (Paraffin sections) (IHC-P). GTX637916

目录号 GTX637916

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2015

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Zebrafish, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 HPRT 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

25 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human HPRT

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

hypoxanthine phosphoribosyltransferase 1 , HGPRT , HPRT

细胞定位

Cytoplasm

功能与背景

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

数据库

研究领域

產品图片

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

KO/KD

Wild-type (WT) and HPRT1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637916

GTX637916 ICC/IF Image

HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HPRT antibody [HL2015] used in IHC (Paraffin sections) (IHC-P). GTX637916

GTX637916 IHC-P Image

HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in IHC (Paraffin sections) (IHC-P). GTX637916

GTX637916 IHC-P Image

HPRT antibody [HL2015] detects HPRT protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat intestine.
HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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