产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse liver , 293T , A431 , HeLa , HepG2
预测种属反应
Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.06 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2037194
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
hypoxanthine phosphoribosyltransferase 1 , HGPRT , HPRT
细胞定位
Cytoplasm
功能与背景
HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]
数据库
研究领域
產品图片
|
GTX113466 WB Image
Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX113466 WB Image
HPRT antibody detects HPRT1 protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
12% SDS-PAGE
HPRT antibody (GTX113466) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113466 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113466 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX113466 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT(GTX113466) antibody at 1:200 dilution.
|
|
|
GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.HPRT stained by HPRT antibody (GTX113466) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.HPRT stained by HPRT antibody (GTX113466) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
文献引用
产品评论
目前没有使用 HPRT antibody (GTX113466) 的产品评论被收录。快发表第一篇产品评论吧！
