产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:5000-1:30000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human HSC70 protein, and it does not cross react with human HSPA1A, HSPA1L, HSPA2, HSPA5, HSPA6, and HSPA9 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human HSC70.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71
细胞定位
Cytoplasm,Melanosome,Cell membrane,Nucleolus
功能与背景
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
数据库
研究领域
產品图片
![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/templates/default/js/lightbox/images/loading.gif "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
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GTX637440 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody [HL1788] (GTX637440) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637440 WB Image
Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody [HL1788] (GTX637440) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637440 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody [HL1788] (GTX637440) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637440 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody [HL1788] (GTX637440) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637440 IHC-P Image
HSC70 antibody [HL1788] detects HSC70 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.HSC70 stained by HSC70 antibody [HL1788] (GTX637440) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/upload/website/prouct_img/small/GTX637440/GTX637440_T-44809_20230421_WB_multiple_B_23042500_590.webp "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/upload/website/prouct_img/small/GTX637440/GTX637440_45082_20230630_WB_Z_23062718_635.webp "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/upload/website/prouct_img/small/GTX637440/GTX637440_45082_20230630_WB_23062718_149.webp "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/upload/website/prouct_img/small/GTX637440/GTX637440_T-44809_20230825_WB_M_R_23083020_482.webp "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
![Anti-HSC70 antibody [HL1788] used in IHC (Paraffin sections) (IHC-P). GTX637440](/upload/website/prouct_img/small/GTX637440/GTX637440_T-44809_20230801_IHC-P_M_23081619_481.webp "Anti-HSC70 antibody [HL1788] used in IHC (Paraffin sections) (IHC-P). GTX637440")
![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/upload/website/prouct_img/normal/GTX637440/GTX637440_T-44809_20230421_WB_multiple_B_23042500_590.webp "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/upload/website/prouct_img/normal/GTX637440/GTX637440_45082_20230630_WB_Z_23062718_635.webp "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/upload/website/prouct_img/normal/GTX637440/GTX637440_45082_20230630_WB_23062718_149.webp "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
![Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440](/upload/website/prouct_img/normal/GTX637440/GTX637440_T-44809_20230825_WB_M_R_23083020_482.webp "Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440")
![Anti-HSC70 antibody [HL1788] used in IHC (Paraffin sections) (IHC-P). GTX637440](/upload/website/prouct_img/normal/GTX637440/GTX637440_T-44809_20230801_IHC-P_M_23081619_481.webp "Anti-HSC70 antibody [HL1788] used in IHC (Paraffin sections) (IHC-P). GTX637440")
