HSC70抗体 [N2C1], Internal

目录号 GTX111069

文献引用 3

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P
种属反应	Human, Mouse
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 HSC70 的产品

View product citations for antibody GTX111069 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

71 kDa. ( Note )

阳性对照

Neuro2A , GL261 , C8D30 , A431 , HeLa , 293T , HepG2

预测种属反应

Rat, Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950526

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71

细胞定位

Cytoplasm , Melanosome

功能与背景

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

数据库

Gene ID: 3312 HSPA8
UniProt: P11142 HSPA8

研究领域

產品图片

GTX111069 WB Image

Hsc70 antibody detects Hsc70 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Hsc70 antibody (GTX111069) dilution: 1:5000

GTX111069 IHC-P Image

HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HSC70 antibody (GTX111069) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111069 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70(GTX111069) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111069 ICC/IF Image

HSC70 antibody detects HSC70 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSC70 protein stained by HSC70 antibody (GTX111069) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX111069 WB Image

Sample (30 μg of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX111069 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111069 IHC-P Image

GTX111069 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: HeLa
7.5% SDS PAGE
GTX111069 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Fussi N et al. Cell Death Dis 2018; 9 (7) : 757 Exosomal secretion of α-synuclein as protective mechanism after upstream blockage of macroautophagy.

实验应用 : WB

种属反应 : Human

Soliman M et al. PLoS Pathog 2018; 14 (1) : e1006820 Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.

实验应用 : WB

种属反应 : Human

Luu BE et al. Cell Stress Chaperones 2017; The regulation of heat shock proteins in response to dehydration in Xenopus laevis.

实验应用 : WB

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产品评论

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Application Reference

Fussi N et al. Cell Death Dis 2018; 9 (7):757 Exosomal secretion of α-synuclein as protective mechanism after upstream blockage of macroautophagy.

实验应用 : WB

种属反应 : Human

Soliman M et al. PLoS Pathog 2018; 14 (1):e1006820 Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.

实验应用 : WB

种属反应 : Human

Luu BE et al. Cell Stress Chaperones 2017; The regulation of heat shock proteins in response to dehydration in Xenopus laevis.

实验应用 : WB

SDS
Glycerol.pdf
Thimerosal.pdf

产品包装

报价 (¥)

100 μl

¥ 4000

25 μl

¥ 1700

Qty

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	http://www.neobioscience.com
	genetex@neobioscience.com

HSC70抗体 [N2C1], Internal

目录号 GTX111069

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX111069 WB Image

GTX111069 IHC-P Image

GTX111069 IHC-P Image

GTX111069 ICC/IF Image

GTX111069 WB Image

GTX111069 IHC-P Image

GTX111069 WB Image

文献引用

Application Reference

产品评论

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