产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa (25 μg/ml Anisomycin treatment for 30 min)
预测种属反应
Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Ser82 of human HSP27. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888450
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27
细胞定位
Cytoplasm , Nucleus , Spindle
功能与背景
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/templates/default/js/lightbox/images/loading.gif "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
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GTX634421 WB Image
Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634421 ICC/IF Image
HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX634421 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634421 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/small/GTX634421/GTX634421_43024_20190823_WB_treatment_M_CIP_w_23061202_951.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421](/upload/website/prouct_img/small/GTX634421/GTX634421_43024_20190717_ICC_IF_w_23061202_123.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/small/GTX634421/GTX634421_43024_20190809_WB_R_w_23061202_292.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/small/GTX634421/GTX634421_43024_20171117_WB_Anisomycin_w_23061202_746.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/normal/GTX634421/GTX634421_43024_20190823_WB_treatment_M_CIP_w_23061202_951.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421](/upload/website/prouct_img/normal/GTX634421/GTX634421_43024_20190717_ICC_IF_w_23061202_123.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/normal/GTX634421/GTX634421_43024_20190809_WB_R_w_23061202_292.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/normal/GTX634421/GTX634421_43024_20171117_WB_Anisomycin_w_23061202_746.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
