Haploid sperm cell-specific antigen抗体 [TRA54]

Name: Haploid sperm cell-specific antigen抗体 [TRA54]
Brand: GeneTex
SKU: GTX00716
Price: 5600.00 RMB

目录号 GTX00716

引用文献 3

目录号 GTX00716

GTX00716 IHC-P Image

IHC-P analysis of mouse testis tissue section (C57BL/6) using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54].
Fixation : Bouin's solution

1 / 3

GTX00716 IHC-P Image

IHC-P analysis of mouse testis tissue section (8-month) using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54]. Anti-GATA antibody stains nuclei of Sertoli cells in seminiferous tubule while anti-TRA54 antibody stains reproductive cells.
Dilution : 1:50
Antigen retrieval : 10 mM citrate buffer (pH 6.0) for 15min
Fixation : 4% PFA

2 / 3

GTX00716 WB Image

WB analysis of various mouse tissue samples using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54].
Dilution : 1 μg/ml
Loading : 100 μg

3 / 3

宿主

Rat
克隆

Monoclonal
克隆编号

TRA54
同种型

IgM
实验应用

WB ICC/IF IHC-P IHC-Fr IP Dot EM
种属反应

Mouse, Rat

摘要

Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification. The molecular size diversity is due to the difference in glycosylation.