产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 0.5-2μg/ml |
| 5μg/ml |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
The peptide used to generate this antibody is available for purchase (GTX23393-PEP).
产品属性
形式
Liquid
存储溶液
TBS, 0.5% BSA
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Peptide with sequence C-YEKKATLIKSSEE, from the C Terminus of the protein sequence according to NP_006092.1.
纯化方式
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
细胞定位
Nucleus
功能与背景
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
数据库
研究领域
產品图片
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GTX23393 IHC-P Image
IHC-P analysis of human adrenal gland using GTX23393 Hec1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX23393 WB Image
WB analysis of HeLa lysate using GTX23393 Hec1 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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GTX23393 IHC-P Image
IHC-P analysis of human liver using GTX23393 Hec1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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文献引用
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