应用说明
Recommended Starting Dilutions:
For WB: Use at a dilution of 1:300
For ICC/IF: Use at a dilution of 1:150
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
预期分子量
阳性对照
HeLa , Blocking peptide (GTX70013-PEP)
形式
Liquid
存储溶液
1X PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.127 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic phosphopeptide (GTX70013-PEP) corresponding to the amino acids around Ser 165 conjugated to KLH
纯化方式
Affinity purified using antigen
偶联
Unconjugated
RRID
AB_11174082
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
别名
NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
细胞定位
Nucleus
功能与背景
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
数据库
研究领域