产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human Heme Oxygenase 1 protein, and it does not cross react with human Heme Oxygenase 2 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human Heme Oxygenase 1
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10
细胞定位
Microsome,Endoplasmic reticulum membrane
功能与背景
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/templates/default/js/lightbox/images/loading.gif "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
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GTX637432 WB Image
Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637432 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637432 IHC-P Image
Heme Oxygenase 1 antibody [HL1780] detects secreted Heme Oxygenase 1 protein by immunohistochemical analysis.Sample: Paraffin-embedded cat spleen.Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637432 IHC-P Image
Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat spleen.Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637432 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637432 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637432 IHC-P Image
Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/upload/website/prouct_img/small/GTX637432/GTX637432_T-44802_20221209_WB_KO_watermark_22121123_519.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/upload/website/prouct_img/small/GTX637432/GTX637432_44935_20230203_WB_treatment_CoCl2_23020621_526.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432](/upload/website/prouct_img/small/GTX637432/GTX637432_44935_20230714_IHC-P_Cat_23081619_746.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432](/upload/website/prouct_img/small/GTX637432/GTX637432_T-44802_20230714_IHC-P_R_23071822_211.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/upload/website/prouct_img/small/GTX637432/GTX637432_T-44802_20221216_WB_multiple_B_22122018_149.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/upload/website/prouct_img/small/GTX637432/GTX637432_44935_20230616_WB_D_23062019_216.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432](/upload/website/prouct_img/small/GTX637432/GTX637432_T-44802_20230714_IHC-P_M_23071822_770.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/upload/website/prouct_img/normal/GTX637432/GTX637432_T-44802_20221209_WB_KO_watermark_22121123_519.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/upload/website/prouct_img/normal/GTX637432/GTX637432_44935_20230203_WB_treatment_CoCl2_23020621_526.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432](/upload/website/prouct_img/normal/GTX637432/GTX637432_44935_20230714_IHC-P_Cat_23081619_746.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432](/upload/website/prouct_img/normal/GTX637432/GTX637432_T-44802_20230714_IHC-P_R_23071822_211.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/upload/website/prouct_img/normal/GTX637432/GTX637432_T-44802_20221216_WB_multiple_B_22122018_149.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432](/upload/website/prouct_img/normal/GTX637432/GTX637432_44935_20230616_WB_D_23062019_216.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432")
![Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432](/upload/website/prouct_img/normal/GTX637432/GTX637432_T-44802_20230714_IHC-P_M_23071822_770.webp "Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432")
