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Heme Oxygenase 1抗体

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in ELISA (ELISA). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

目录号 GTX101147

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

种属反应

Human, Mouse, Rat, Monkey
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX633677, Detection: GTX101147

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

33 kDa. ( Note )

阳性对照

HeLa,U87-MG,NIH-3T3

预测种属反应

Bovine, Dog, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950502

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10

细胞定位

Microsome , Endoplasmic reticulum

功能与背景

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101147

GTX101147 ICC/IF Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human renal carcinoma .
Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in ELISA (ELISA). GTX101147

GTX101147 ELISA Image

Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 μg/mL and GTX101147 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the journal Int J Mol Sci in 2019. PMID: 30621167

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the journal Cells in 2019. PMID: 31366086

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the journal Cell Death Dis in 2017.PMID: 28055010

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911

文献引用

产品评论

Heme Oxygenase 1 antibody 目录号 GTX101147

Rating ( Average 5 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Normal spleen tissue
Fixation : EDTA buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
Mariko Kogami et al. Oncol. Lett. 2023; 26:313
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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